动物营养学报Issue(2)：489-495,7.DOI:10.3969/j.issn.1006-267x.2014.02.026
硫胺素对高精料底物瘤胃体外发酵培养液挥发性脂肪酸和乳酸浓度的影响
Effects of Thiamine on Concentrations of Volatile Fatty Acids and Lactate in Culture Medium of High Concentrate Substrate after in Vitro Rumen Fermentation
摘要
Abstract
This experiment was conducted to study the effects of thiamine on concentrations of volatile fatty acids and lactate in culture medium of high concentrate substrate after in vitro rumen fermentation.Four healthy Holstein dairy cows equipped with permanent ruminal cannulas were used to provide rumen fluid.The fermen-tation substrate was consist of casein, wheat starches, soluble starches, pectins, xylan, milled filter paper and carboxymethyl cellulose, and the concentrate to forage ratio was 6∶4.The supplemental levels of thiamine were 0, 60, 120, 180 and 240 mg/kg, respectively.The results showed as follows:generally, the lactate concen-tration in culture medium was decreased by thiamine under the condition of high concentrate substrate, and 180 mg/kg group showed the best effect.It was also observed that 180 mg/kg group increased total volatile fatty acid concentration but decreased the ratio of acetate to propionate.It is concluded that the supplemental level of 180 mg/kg thiamine can stabilize rumen microbial fermentation of high concentrate substrate with 6∶4 of the concentrate to forage ratio.关键词
硫胺素/瘤胃发酵/乳酸/挥发性脂肪酸Key words
thiamine/rumen fermentation/lactate/volatile fatty acid分类
农业科技引用本文复制引用
张军,王梦芝,郝志敏,喻礼怀,王洪荣..硫胺素对高精料底物瘤胃体外发酵培养液挥发性脂肪酸和乳酸浓度的影响[J].动物营养学报,2014,(2):489-495,7.基金项目
国家自然科学基金项目（31072051）；江苏省自然科学基金项目（BK2011447）；江苏省优势学科 （）
