南方医科大学学报Issue(10)：1471-1473,3.
小白菊内酯对高糖诱导的大鼠肾小球系膜细胞增殖及核因子-κB、单核细胞趋化蛋白-1表达的影响
Effects of parthenolide on high glucose-induced cell proliferation, NF-κB activation and MCP-1 expression in rat mesangial cells
摘要
Abstract
Objective To explore the effects of parthenolide (PTL) on high glucose-stimulated cell proliferation, NF-κB activation and monocyte chemoattractant protein-1 (MCP-1) expression in rat mesangial cells (MCs). Methods MCs were cultured in normal glucose (5.6 mmol/L), high glucose (30 mmol/L), or high glucose with PTL. MTT assay was used to detect the cell proliferation. MCP-1 content in the supernatant was measured by ELISA, and the level of IκBα was detected by Western blotting to reflect NF-κB activity. EMSA method was used to measure the activation of NF-κB. Results MC proliferation, MCP-1 expression and NF-κB activation were significantly enhanced and the expression of NF-κB-binding protein IκBα was obviously reduced in cells cultured in high glucose. Application of PTL obviously abolished the effects of high glucose. Conclusion PTL can suppress high glucose-stimulated NF-κB activation and MCP-1 expression in rat MC. These data provide a theoretical basis for the clinical application of PTL in prevention and control of diabetic nephropathy.关键词
小白菊内酯/糖尿病肾病/核因子-κB/单核细胞趋化蛋白-1Key words
parthenolide/diabetic nephropathy/nuclear factor kappa B/monocyte chemoattractant protein-1引用本文复制引用
许兆忠,贾倩倩,李红瑜,王建成,赵艳,陈斯佳,龙海波..小白菊内酯对高糖诱导的大鼠肾小球系膜细胞增殖及核因子-κB、单核细胞趋化蛋白-1表达的影响[J].南方医科大学学报,2013,(10):1471-1473,3.基金项目
国家自然科学基金（81072848）；国家级大学生创新创业训练计划项目（201212121021）；广东省科技计划项目（2011B061300014） Supported by National Natural Science Foundation of China (81072848) （81072848）
