南方医科大学学报Issue(10)：1483-1488,6.
利用Agilent定制基因芯片筛选相同病理类型、不同预后的早期乳腺癌的分子标记物
Screening molecular markers in early breast cancer of the same pathological types but with different prognoses using Agilent gene chip
摘要
Abstract
Objective:To screen molecular markers in early breast cancer and establish gene subtyping-based diagnostic criteria for predicting the prognosis of early breast cancers. Methods Tumor tissue specimens were obtained from 8 patients with early breast cancer for analysis of the differentially expressed genes using Agilent custom 8 × 15 000 chips in combination with the prognostic data of the patients. Another 42 tumor tissue specimens were used to validate the differential genes by real-time fluorescent quantitative PCR. Results Gene microarray analysis identified 132 differentially expressed genes between the patients with favorable and poor prognosis, and 44 of these genes were significantly up-regulated (by over two folds) and 88 down-regulated in patients with poor prognoses. Conclusion The gene expression profiles differ in early breast cancer tissues of the same pathological type but with different clinical stages and prognoses, and CD44, MKI67, NTRK2, Nek2, C16orf60, TOP2A, ANCCA, and RRM2 genes can be used as the prognostic markers for early breast cancer.关键词
乳腺肿瘤/基因/芯片分析技术/分子分型Key words
breast tumors/genes/chip analysis techniques/molecular typing引用本文复制引用
厉周,金维荣,彭亮,韩帅,黄宗海,史福军,蔡寨,李秀勤,张普生,朱卉娟..利用Agilent定制基因芯片筛选相同病理类型、不同预后的早期乳腺癌的分子标记物[J].南方医科大学学报,2013,(10):1483-1488,6.基金项目
广东省科技计划项目（2007B030703006）；广州市海珠区科普项目 （D）
