南方医科大学学报Issue(10)：1504-1507,4.
凋亡细胞预处理对胰岛移植受体大鼠脾淋巴细胞功能的影响
Effect of pretreatment with apoptotic donor spleen cells on spleen lymphocyte function of recipient rats after islet transplantation
摘要
Abstract
Objective To study the effect of pretreatment with apoptotic donor spleen cells on spleen lymphocyte function of recipient rats undergoing islet transplantation to explore new approaches to prolong islet graft survival. Methods Apoptotic spleen cells from donor rats were obtained by exposure toγ-ray irradiation from 60Co. Diabetic SD rat models were randomly divided into 4 groups to receive tail vein injections with saline (group A), normal cells (group B), apoptotic donor cells (group C), or necrotic donor cells (group D). One week later, orthotopic transplantation of islets under the renal capsule was performed. Before and at 1 and 2 weeks after islet transplantation, the recipient rats were examined for proliferative activity of spleen lymphocytes with CFSE cell staining and for IL-2 and IL-10 expressions in the cells using ELISA. Results Pretreatment with donor apoptotic cells significantly suppressed the proliferative activity of recipient spleen lymphocytes before and at 1 and 2 weeks after islet transplantation as compared with the other three groups (P<0.05). The level of IL-2 was significantly decreased while IL-10 increased in apoptotic donor cell pretreatment group compared with those in the other 3 groups at each time point of observation. Conclusion The effect of pretreatment with apoptotic donor cells on recipient spleen lymphocytes suggest an important role of apoptotic donor spleen cells in immune tolerance of grafts.关键词
凋亡/胰岛移植/淋巴细胞增殖/淋巴细胞因子Key words
apoptosis/islet transplantation/lymphocytes proliferation/immune tolerance引用本文复制引用
李双喜,陈宏,杨蕾,陈容平,张桦,蔡德鸿,张振..凋亡细胞预处理对胰岛移植受体大鼠脾淋巴细胞功能的影响[J].南方医科大学学报,2013,(10):1504-1507,4.基金项目
国家自然科学基金（30470829） Supported by National Natural Science Foundation of China(30470829) （30470829）
