南方医科大学学报Issue(11)：1685-1688,4.
小剂量氯胺酮预处理对大鼠气腹所致小肠缺血再灌注损伤的保护作用
Protective effect of low-dose ketamine against intestinal ischemia-reperfusion injury following carbon dioxide pneumoperitoneum in rats
摘要
Abstract
Objective To investigate the protective effect of low-dose ketamine against intestinal ischemia reperfusion injury following pneumoperitoneum with carbon dioxide in rats. Methods Thirty healthy male adult SD rats (body weight 280-320 g) were randomized into sham-operated group, model group and ketamine group and subjected to pneumoperitoneum for 120 min with carbon dioxide (not in sham-operated group). The rats in ketamine group received an intraperitoneal injection of 10 mg/kg ketamine 10 min before pneumoperitoneum, and those in the other two groups received saline injection. Fifteen minutes after pneumoperitoneum or sham operation, the small intestines were sampled to detect the content of malondialdehyde (MDA) and fore pathological testing. ELISA was used to detect the serum levels of I-FABP, TNF-a IL-6 and IL-8. Results Pneumoperitoneum caused a significant increase in intestinal MDA content (P<0.05), which was lowered by ketamine pretreatment (P<0.05). Serum I- FABP, TNF- a, IL- 6 and IL- 8 levels all significantly increased following pneumoperitoneum (P<0.05) and were obviously lowered by ketamine pretreatment (P<0.05). Pneumoperitoneum also caused obvious pathologies in intestinal mucosa, which were ameliorated by ketamine pretreatment. Conclusion Low-dose ketamine preconditioning can reduce the inflammatory reaction and lessen oxidative damage in the intestinal mucosa following pneumoperitoneum in rats.关键词
小剂量氯胺酮预处理/气腹/小肠/缺血再灌注损伤Key words
low-dose ketamine preconditioning/pneumoperitoneum/small intestine/ischemia reperfusion injury引用本文复制引用
王伟,田福宏,严六狮..小剂量氯胺酮预处理对大鼠气腹所致小肠缺血再灌注损伤的保护作用[J].南方医科大学学报,2013,(11):1685-1688,4.基金项目
陕西省科技攻关项目 （）
