南方医科大学学报Issue(1)：46-50,5.
过表达Wnt3基因抑制肝前体细胞凋亡
Overexpression of Wnt3 inhibits apoptosis of hepatic progenitor cells in vitro
摘要
Abstract
Objective To investigate the effects of adenoviral vector-mediated over-expression of Wnt3 on the apoptosis of hepatic progenitor cells in vitro. Methods Hepatic progenitor cells transfected with Ad-GFP-Wnt3 vector or the control vector Ad-GFP were examined for cell apoptosis under fluorescence microscopy with Hoechst 33342 staining, and the proportion of apoptotic cells were determined by flow cytometric analysis with Annexin-PE/7-ADD staining. The mRNA and protein expressions of Bax, Bcl-2 and Bcl-xl in the cells were detected by real-time PCR and Western blotting, respectively. Results Real-time PCR and Western blotting showed a high expression of Wnt3 in Ad-GFP-Wnt3-transfected hepatic progenitor cells, which exhibited significantly decreased cell apoptosis as compared with the control group. The expressions of Bcl-2 and Bcl-xl mRNA and proteins increased significantly while Bax expression decreased obviously in Ad-GFP-Wnt3-transfected cells (P<0.05). Conclusion Adenoviral vector-mediated over-expression of Wnt3 can suppress apoptosis of hepatic progenitor cells possibly through the Bcl-2 pathway.关键词
肝前体细胞/抗凋亡/Wnt3Key words
hepatic progenitor cells/cell apoptosis/Wnt3引用本文复制引用
张锡峰,胡代曦,张超,钟沁,冯涛,黄佳祎..过表达Wnt3基因抑制肝前体细胞凋亡[J].南方医科大学学报,2014,(1):46-50,5.基金项目
国家自然科学基金（81071770，81201679）@@@@Supported by National Natural Science Foundation of China (81071770,81201679) （81071770,81201679）
