南方医科大学学报Issue(2)：180-187,8.
胃癌中幽门螺杆菌L型感染与MIF、MMP9、VEGF表达的关系
Infection with L-form of Helicobacter pylori and expressions of MIF, MMP9 and VEGF in gastric carcinoma
摘要
Abstract
Objective To investigate the correlation between infection with L-form of Helicobacter pylori (Hp-L) and the expressions of macrophage migration inhibition factor (MIF), matrix metalloproteinase 9 (MMP9), and vascular endothelial growth factor (VEGF) in gastric cancer. Methods Hp-L was examined in 80 gastric carcinoma and 50 adjacent normal tissues by Gram staining and immunohistochemical staining, and the expressions of MIF, MMP9 and VEGF were detected by immunohistochemical staining;the expression of MIF mRNA was detected by RT-PCR and the expression of MIF, MMP9 and VEGF proteins were detected by Western blotting in 30 fresh gastric cancer tissues and the corresponding adjacent tissues. Results Of the 80 gastric carcinoma tissues, 57 (71.25%) showed Hp-L positivity detected by both Gram staining and immunohistochemical staining, as compared with a rate of only 14% in the adjacent normal tissues (P<0.05). The gastric carcinoma tissues showed higher expression levels of MIF, MMP9 and VEGF proteins than the corresponding adjacent normal mucosa;the positivity MIF, MMP-9 and VEGF proteins were significantly higher in Hp-L-positive gastric carcinoma than in Hp-L-negative cases (P<0.05). Positive correlations were found between Hp-L positivity and the expressions of MIF, MMP-9 and VEGF (r=0.598, 0.292, 0.341, respectively, P<0.05). The 30 fresh gastric cancer tissues showed also significantly higher MIF mRNA expression and MIF, MMP-9 and VEGF protein expressions than the adjacent tissues (t=3.729, P<0.01). The expressions of MIF and MMP-9 were also related to the clinicopathological factors including lymph node metastasis and depth of invasion (P<0.05). Conclusion Infection with L-form of Hp-L can be an important factor that contributes to the invasion and metastasis of gastric carcinoma, the mechanism of which involves up-regulated expressions of MIF, MMP-9 and VEGF.关键词
胃癌/幽门螺杆菌/细菌L型/巨噬细胞移动抑制因子/基质金属蛋白酶-9/血管内皮生长因子Key words
gastric carcinoma/Helicobacter pylori/bacterial L- forms/macrophage migration inhibition factor/matrix metalloproteinase 9/vascular endothelial growth factor引用本文复制引用
欧玉荣,康敏,周蕾,承泽农,唐素兰,于东红..胃癌中幽门螺杆菌L型感染与MIF、MMP9、VEGF表达的关系[J].南方医科大学学报,2014,(2):180-187,8.基金项目
安徽省教育厅自然科学研究项目 （）
