南方医科大学学报Issue(2)：223-227,5.
自体猪血体外常温持续灌流离体肝脏的可行性分析
Feasibility of continuous extracorporeal normothermic liver perfusion using autologous blood:a study in pigs
摘要
Abstract
Objective To study the feasibility of sustaining the viable status of a liver graft in at least 96 h by extracorporeal perfusion using autologous blood. Methods Eight extracorporeal porcine liver perfusions using autologous blood were performed, each for 96 h with hepatectomy, cold preservation, cannulation of vessels, and initiation of perfusion with normothermic oxygenated porcine blood. The graft viability was assessed by metabolic, synthetic, hemodynamic, and histologic parameters. Results After 96 h of normothermic, extracorporeal perfusion using autologous blood, the isolated livers maintained normal physiological levels of pH and electrolytes with sustained hepatic protein synthesis (complement and factor V) throughout the perfusion. Hemodynamic parameters maintained normal physiological ranges. Histological inspection demonstrated good preservation of the liver with a good architectural integrity. Conclusion It is possible to sustain the viable status of a liver graft within 96 h by extracorporeal perfusion using autologous blood.关键词
猪肝/自体血/体外灌流/肝衰竭/器官保存Key words
porcine liver/autologous blood/extracorporeal perfusion/hepatic failure/organ preservation引用本文复制引用
向开敏,禹华旭,袁金忠,李征..自体猪血体外常温持续灌流离体肝脏的可行性分析[J].南方医科大学学报,2014,(2):223-227,5.基金项目
国家自然科学基金（81171132）；湖南省自然科学基金（04JJ6017）；湖南省教育厅重点课题（10A013）；湖南省重点学科建设经费资助 （）
