南方医科大学学报Issue(4)：492-496,5.DOI:10.3969/j.issn.1673-4254.2014.04.11
活性维生素D3下调屋尘螨诱导的气道上皮细胞胸腺基质淋巴生成素蛋白释放
1,25-dihydroxyvitamin D3 pretreatment inhibits house dust mite-induced thymic stromal lymphopoietin release by human airway epithelial cells
摘要
Abstract
Objective To investigate the effect of 1,25-dihydroxyvitamin D3 (1,25VD3) on house dust mites (HDM)-induced expression of thymic stromal lymphopoietin (TSLP) in human airway epithelial cells in vitro. Methods Human airway epithelial 16HBE cells were incubated with 200, 400, and 800 U/L in the absence or presence of 1,25VD3 (10-8 mol/L) for 6 h and 24 h, and TSLP mRNA and protein expressions in the cells were assessed using quantitative PCR and ELISA. Results 16HBE cells incubated with HDM at 200, 400, and 800 U/L showed significantly increased TSLP mRNA and protein expressions (P<0.05). Pretreatment of the cells with 1,25VD3 obviously lowered 400 U/L HDM-induced TSLP expressions (P<0.05), but 1,25VD3 added along with HDM in the cells did not produce significant effects on TSLP expressions (P=0.58). Conclusion Both 1,25VD3 and HDM can induce TSLP expression and release in 16HBE cells, but pretreatment with 1,25VD3 can decrease HDM-augmented TSLP expression in the cells.关键词
屋尘螨/胸腺基质淋巴生成素/气道上皮细胞/活性VD3Key words
house dust mite/thymic stromal lymphopoietin/human airway epithelial cells/1,25-dihydroxyvitamin D3引用本文复制引用
周丽芹,董航明,赵海金,邹梦晨,姚利红,邹飞,蔡绍曦..活性维生素D3下调屋尘螨诱导的气道上皮细胞胸腺基质淋巴生成素蛋白释放[J].南方医科大学学报,2014,(4):492-496,5.基金项目
国家自然科学基金（81270087,81270089）；国家重点基础研究发展计划（“973”计划）（2012CB518200）；广东省教育部产学研结合项目(2012B091100153)Supported by National Natural Science Foundation of China(81270087,81270089) and National Basic Research Program (973 Program)(2012CB518200) （2012B091100153）
