南方医科大学学报Issue(4)：504-506,510,4.DOI:10.3969/j.issn.1673-4254.2014.04.14
外源性硫化氢对高糖条件下原代神经元β淀粉样蛋白的影响
Effect of exogenous hydrogen sulfide on BACE-1 enzyme expression and β-amyloid peptide metabolism in high-glucose primary neuronal culture
摘要
Abstract
Objective To investigate the effects of exogenous hydrogen sulfide (H2S) onβ-site APP cleaving enzyme 1 (BACE-1) and β-amyloid peptide (Aβ) metabolism in primary culture of neurons under high-glucose condition. Methods The cortical neurons in primary culture under normal and high glucose (60 mmol/L) conditions for 24 h were exposed to 25, 50 and 100μmol/L NaHS. Aβ1-42 concentration in the cell culture was measured by ELISA, and BACE-1 mRNA and protein levels were detectedby fluorescent quantitative real-time PCR and Western blotting, respectively. Results Compared with the neurons cultured in normal glucose, the neurons exposed to high glucose showed significantly increased Aβ1-42 concentration and BACE-1 mRNA and protein expressions (P<0.05). Exposure to 25, 50 and 100 μmol/L NaHS significantly decreased Aβ1-42 concentration and BACE-1 mRNA and protein expressions in the high-glucose cell culture (P<0.05). Conclusion Neurons exposed to high glucose exhibit increased Aβ1-42 levels and BACE-1 mRNA and protein expressions, which can be concentration-dependently decreased by NaHS.关键词
硫化氢/原代神经元/高糖环境/淀粉样前体蛋白裂解酶-1/β淀粉样蛋白Key words
hydrogen sulfide/primary culture neuron/high glucose environment/β-site APP cleaving enzyme 1/β-amyloid peptide metabolism引用本文复制引用
朱丽娟,陈筱山,何选丽,戚韵雯,晏勇..外源性硫化氢对高糖条件下原代神经元β淀粉样蛋白的影响[J].南方医科大学学报,2014,(4):504-506,510,4.基金项目
国家自然科学基金（81271222）Supported by National Natural Science Foundation of China (81271222) （81271222）
