福建林学院学报Issue(2)：152-157,6.
叶子花花色DOD基因全长cDNA克隆与序列分析
Cloning and sequence analysis of full-length cDNA of the Bougainvillea spectabilis Willd.flower color DOD gene
摘要
Abstract
The RNA of Bougainvillea spectabilis Willd.bracts were extracted , and the 4, 5-DOPA dioxygenase ( DOD ) gene full-length cDNA was cloned by RACE technique .The full length of the cDNA was 1 059 bp, open reading frame ( ORF) was located between the 56-802 bp,which encoding 249 amino acid residues .According to bioinformatic analytical results of the amino acid sequence , there was 4,5-DOPA dioxygenase conserved domain in this sequence , and the molecular weight of the protein com-ponent was 27.94 kD.The pI was 5.48, and it was stable protein.The prediction of the secondary structure showed that there were 8 α-coils, 8 β-sheets, and 20β-turns in it.Most of the amino acid residues were hydrophilic , yet no signal peptide and transmem-brane structure was found .The blast results showed that as the differences of the species increased , the similarity of the DOD was decreased .It is indicated that DOD gene was quite conserved in betalain plants by discovering the phenomenon that anthocyanins metabolism and betalain metabolism were different and could not coexist in one plant .关键词
叶子花/多巴双加氧酶/甜菜色素/基因克隆Key words
Bougainvillea spectabilis Willd./4,5-DOPA dioxygenase/betalain/gene cloning分类
生物科学引用本文复制引用
柏忠良,佘文琴,潘东明,郭怀攀..叶子花花色DOD基因全长cDNA克隆与序列分析[J].福建林学院学报,2014,(2):152-157,6.基金项目
福建农林大学创新团队“园艺植物种质与高优生产技术创新”（ cxtd12013）。 （）
