福建林业科技Issue(1)：63-67,120,6.DOI:10.13428/j.cnki.fjlk.2014.01.014
基于线粒体 COI 基因鉴定大小蠹属昆虫
Identification of Dendrotonus Insects Based on the Mitochondrial COI Gene
摘要
Abstract
Taking Dendrotonus valens,D.pseudotsug,D.ponderosae,D.adjunctus,D.rufipennis and D.simplex 6 Dendrotonus species often intercepted in ports as the smaples,this paper constructed a Close Sib Relationship Tree;it used molecule measures to amplify mitochondrial COI genes,it analysed the relationships among same source sequence diversity,heredity distance and system evolution, and Close Sib Relationship Tree and Molecule Evolution Tree were compared,and it researched the feasibility and accuracy of Dend-rotonus insects rapid identification with the molecular techniques.The results showed that the homogeneous Dendrotonus bases had the small difference and different kinds of it had the larger difference,the different species could be clearly distinguished;the Dendro-tonus genetic distance and Neighbour-Joining System Evolution Tree also confirmed that the molecular identification results and the retrieval table classification results were the same.The molecular identification methods were used to do the fast accurate identifica-tion for different kinds of Dendrotonus insects.关键词
大小蠹属/形态学/COI基因/分子鉴定Key words
Dendrotonus/morphology/COI gene/molecular identification分类
农业科技引用本文复制引用
殷玉生,张帆,安榆林..基于线粒体 COI 基因鉴定大小蠹属昆虫[J].福建林业科技,2014,(1):63-67,120,6.基金项目
科技部质检公益性行业科研专项（201110038）；江苏出入境检验检疫局基金课题 （）
