福建农业学报Issue(9):859-863,5.
嗜水气单胞菌外膜蛋白 ISCOM 疫苗免疫欧洲鳗鲡后抗体分泌细胞变化
European Eel Antibody Secreting Cells Changes After Immunize by Aeromonas hydrophila MOMP ISCOM Vaccine
摘要
Abstract
Aeromonas hydrophila MOM P-ISCOMs vaccine was prepared , and the vaccine was given to European eel by oral or injection .The European eel skin and kidney cells were separated and isolated after immunization 14 and 28 days respectively ,in which the antibody secreting cells (ASC) ,were analyzed by ELISPOT technique .The results showed that the two immune ways could affect the number of immune cells in skin and kidneys in different amount of non-specific immune cells increased rapidly in oral immunization group , and specific immune cells increased more obviously in injection group .关键词
嗜水气单胞菌/欧洲鳗鲡/外膜蛋白ISCOMs疫苗/皮肤/肾脏/ASCKey words
Aeromonas hydrophila/europeaneel/MOMP ISCOM vaccine/skin/kidney/ASC分类
农业科技引用本文复制引用
林晨韬,黄春兰,李艳虹,李盼,宋铁英..嗜水气单胞菌外膜蛋白 ISCOM 疫苗免疫欧洲鳗鲡后抗体分泌细胞变化[J].福建农业学报,2013,(9):859-863,5.基金项目
福建省自然科学基金项目(2011J05054);福建省科技重大专项(2010NZ0002-3);福建省农业科学院引进海外人才科研启动基金项目 ()