福建农业学报Issue(10):976-980,5.
茉莉花愈伤组织的诱导及其继代保持
Induction,Subculture and Maintenance of Fuzhou Jasmine, Jasminum sambac (Linn .) Aiton
摘要
Abstract
Plantlets of Fuzhou jasmine , Jasminum sambac (Linn .) Aiton ,were obtained by using the stems and floral organ of the plant as explants . The callus induction ,subculture and long-term maintenance of the plantlets were investigated on media with varied concentrations of phytohormone . The results indicated that the optimal light density for the culture was 1 500 -2 000 lx ,and that 6 different types of callus could be formed by varying the phytohormone concentration . The medium consisting of MS ,2.0 mg BA/L ,0.2 mg NAA · L -1 and 30 g sucrose · L -1 yielded a 100% induction rate ,and at the same time ,was the best for the callus subculture multiplication ,as well as the growth of the induced plants .关键词
茉莉花/外植体/愈伤组织/诱导/继代保持Key words
jasmine [Jasminum sambac (Linn .) Aiton]/explant/callus/induction/subculture/maintenance分类
农业科技引用本文复制引用
甘煌灿,赖呈纯,朱育菁,陈文辉,李延晖,刘波..茉莉花愈伤组织的诱导及其继代保持[J].福建农业学报,2013,(10):976-980,5.基金项目
福建省农业科学院导师制青年科技创新基金项目 ()