福建农业学报Issue(2):107-112,6.
不同培养条件水滴伪康纤虫种群生长研究
Effects of Different Culture Conditions on the Population Growth of Pseudocohnilembus Persalinus
摘要
Abstract
Three different temperatures (6 ,12 and 20℃) and four culture mediums (lixivium of shrimp flesh , lixivium of wheat , beef extract and peptone culture solution ) were used to reveal the population dynamics of P .persalinus in different conditions .The results showed that :the proliferation rate of the population density at 6℃is slow ,increased with the concentration of culture media ,reached growth period at day 9 and got the maximum on day 12 ;at 20℃ , the population density increased rapidly and the group of beef extract reached the maximum population density (57 490 ind · mL -1 ) on day 3 ;at 12℃ ,the population density showed :beef extract> wheat lixivium > shrimp flesh lixivium> peptone .The largest population density of the optimal concentration of culture solution at 12℃ showed :beef extract (2.25 g · L -1 ) > shrimp flesh (4.5 g · L -1 ) > wheat (10.14 g · L -1 )> peptone (2.25 g · L -1 );the population growth rates showed :beef extract (2.25 g · L -1 ) > wheat (10.14 g · L -1 ) > shrimp flesh (4.5 g · L -1 ) > peptone (2.25 g · L -1 ) ,therefore ,in lixivium of shrimp flesh and wheat the population density was bigger and then the stationary phase was significantly longer than other culture solutions .Our results suggested that beef extract conld be applied to rapidly expanding growth of short-term population ,the lixivium of shrimp flesh and wheat conld be applied to laboratory breeds conservation ,and the population density and population growth rate could reach the maximum at 12℃ and 20℃ ,respectively .These findings will be benefit for the providing of a laboratory culture and preservation method of P .persalinus ,and provide experimental ecological data of P .persalinus in marine aquaculture also .关键词
水滴伪康纤虫/种群密度/种群增长Key words
Pseudocohnilembus persalinus/population density/population growth分类
生物科学引用本文复制引用
沈琳琳,林能锋,詹子锋,曾红..不同培养条件水滴伪康纤虫种群生长研究[J].福建农业学报,2014,(2):107-112,6.基金项目
国家青年科学基金项目(31201698);福建省自然科学基金项目 ()