福建水产Issue(2)：87-95,9.
6株鱼源嗜水气单胞菌的分子鉴定与毒力基因分析检测
Molecular characterization of six strains of Aeromonas hydrophila isolated from fish as well as detection and analysis of virulence genes
摘要
Abstract
Six strains of Aeromonas hydrophila isolated from dying fish were molecularly characterized as well as five virulence genes were detected and analyzed in order to evaluate the feasibility of detecting the virulence genes to infer the potential pathogenicity of A. hydrophila at the molecular lever. 16S rDNA and gyrB gene were used to characterize A. hydrophila,five virulence genes including aerolysin( aer) ,haemolysin( hly) ,serine protease(ahp),heat-stable cytotonic enterotoxin(ast) and heat-labile cytotonic enterotoxin(alt) were de-tected by PCR,and the sequences of gene and virulence were analyzed using Mega 5. 2. All of the strains were characterized as A. hydrophila,and four of five virulence genes at least were detected as positive,including the genes of haemolysin and two kinds of cytotonic enterotoxin. By the sequences analysis, we found the amino acid sequences of high similarity among aerolysin,haemolysin and serine protease. A. hydrophila could be well characterized using 16S rDNA and gyrB gene sequences,and the varied virulence genes indicated the potential pathogenicity. Through the sequences analysis,the genes of aer,hly and ahp were relatively conservative,and the encoding virulences were highly conserved. Detecting the virulence genes of aer,hly and ahp could infer the potential pathogenicity of A. hydrophila in the clinical molecular diagnosis.关键词
嗜水气单胞菌/分子鉴定/gyrB/毒力基因/序列分析Key words
Aeromonas hydrophila/molecular characterization/gyrB/virulence gene/sequence analysis分类
农业科技引用本文复制引用
郭睿,刘年锋,江小斌,张善霹,王伟,郑晨晖,杨小强..6株鱼源嗜水气单胞菌的分子鉴定与毒力基因分析检测[J].福建水产,2014,(2):87-95,9.基金项目
2012年福建省水生动物病害监测计划项目 （）
2012年福州市渔业病虫害防治计划项目 （）
