分析化学Issue(5)：636-642,7.DOI:10.3724/SP.J.1096.2014.30829
液相色谱-高分辨飞行时间质谱法测定食品接触纸包装材料中的7种荧光增白剂
Simultaneous Determination of Seven Fluorescent Whitening Agents in Food Contact Paper Package Materials by High Performance Liquid Chromatography Tandem Triple TOF-High Resolution Mass Spectrometry
摘要
Abstract
A method for screening and quantitative detection of seven fluorescent whitening agents ( FWAs, FWA368 , FWA367 , FWA185 , FWA 184 , FWA52 , FWA135 and FWA393 ) in food contact paper package materials was established by high performance liquid chromatography tandem triple TOF high resolution mass spectrometry ( LC-Triple TOF MS) . The paper samples were extracted with chloroform under ultrasonication, concentrated, separated in a C18 column (100 mmí 2. 1 mm i. d. , 2. 6 μm) by gradient elution using methanol and 0. 1% formic acid in water as mobile phase, and detected in positive ESI and TOF-MS IDA MS/MS mode. The qualitative analysis of seven FWAs was confirmed by synergistic effect of retention time, mass accuracy of precursor, isotope distribution of the precursor, and library search. The analytes were quantified by peak areas of the TOF-MS extracted ions. The results indicated that, all of the seven FWAs presented favorable mass accuracy less than 3í10-6 , good linearity in the concentration of 4-400 μg/L with the correlation coefficients larger than 0. 996, low instrument detection limits ( S/N=3 ) of 1. 0-5. 0 μg/kg, appropriate recoveries ranged from 81. 2% to 98. 9% at three spiked levels, and satisfactory RSD (n=6) of 5 . 2% -9 . 1%. 13 samples were tested using the method and one sample was found FWA 184 with concentration of 287 μg/kg. The method is efficient and accurate, and is suitable for analysis of the seven FWAs in food contact paper package materials.关键词
液相色谱-高分辨飞行时间质谱法/荧光增白剂/食品接触纸包装材料Key words
High performance liquid chromatography tandem triple time of flight-high resolution mass spectrometry/Fluorescent whitening agents/Food contact paper package materials引用本文复制引用
杜志峰,冼燕萍,刘付建,郭新东,黄金凤,吴玉銮,罗海英,王永华..液相色谱-高分辨飞行时间质谱法测定食品接触纸包装材料中的7种荧光增白剂[J].分析化学,2014,(5):636-642,7.基金项目
本文系国家质检总局科技计划资助项目(No.2012QK074) （No.2012QK074）
