分析化学Issue(5)：666-671,6.DOI:10.3724/SP.J.1096.2014.31025
基于共面薄膜金电极的三磷酸腺苷适体传感器
An Aptamer-based Amperometric Biosensor for Adenosine Triphosphate Detection Based on Coplanar Thin-film Gold Electrode
摘要
Abstract
Thin-film gold electrodes were fabricated based on MEMS technology. Adenosine triphosphate with mercapto-group was immobilized on a surface of gold electrode via self-assembly. An aptamer-based amperometric biosensor was constructed to measure the concentration of the ATP that using aptamer as the recognition element based on coplanar thin-film gold electrode. Adenosine triphosphate was detected by the characteristic impedance changes based on phosphate backbone of nucleic with negatively charge that electrostatic reject [ Fe( CN) 6 ] 3-/4-. Firstly, under the conditions of bare gold electrode and before and after ATP was added and whether 6-mercapto-1-hexanol was closed on electrode and the different self-assembly time (3, 8, 15, 24 and 30 h),the electrode of impedance changes in electrochemical impedance solution was researched with electrochemical Impedance Spectroscopy. The experiments show that the measurable linearity range of adenosine triphosphate is 1-500 nmol/L, the detection limit is 1 nmol/L and the correlation coefficient was 0 . 9842 when the time of the self-assembly was 24 h and closed gold electrode with 6-mercapto-hexanol. This electrochemical aptasensor was simplicity and has low detection limit and good repeatability.关键词
共面薄膜金电极/适体传感器/电化学阻抗谱/自组装/三磷酸腺苷Key words
Coplanar thin-film gold electrode/Electrochemical impedance spectroscopy/Aptasensor/Self-assembly/Adenosine triphosphate引用本文复制引用
姜利英,王芬芬,胡杰,岳保磊,闫艳霞,陈青华..基于共面薄膜金电极的三磷酸腺苷适体传感器[J].分析化学,2014,(5):666-671,6.基金项目
本文系国家自然科学基金(No.61002007)、河南省科技创新人才计划(No.124100510001)资助项目 （No.61002007）
