分析化学Issue(5)：766-772,7.DOI:10.3724/SP.J.1096.2014.31026
加压毛细管电色谱-微流蒸发光散射检测器联用系统的构建及其应用
Construction of Microflow Evaporative Light Scattering Detector Coupled with Pressurized Capillary Electrochromatography and Its Application
摘要
Abstract
A novel separation system of microflow evaporative light scattering detector (μELSD) coupled with pressurized capillary electrochromatography ( pCEC) was built and applied to the separation and detection of notoginsenoside R1 , ginsenoside Rg1 , ginsenoside Re, ginsenoside Rb1 , ginsenoside Rd in traditional Chinese medicine extract Xuesaitong (lyophilization). With C18 capillary column the best separation conditions were established by optimizing the mobile phase composition, gradient conditions, flow rate of atomizing gas, evaporating temperature and applied voltage. The mobile phase A was acetonitrile containing 15 mmol/L formic acid-triethylamine acetonitrile ( pH=7 . 0 ) , and the mobile phase B was 15 mmol/L formic acid-triethylamine acetonitrile (pH=7. 0). The optimized gradient elution conditions were as follows:0-10 min, 19%A;10-30 min, 22%A;30-35 min, 36%A;35-45 min, 40%A. The flow rate of atomizing gas( N2 ) was 2 L/min. The evaporating temperature was 120 ℃. The applied voltage was+8 kV. A good linearity was obtained:8. 6-146. 9 ng ( notoginsenoside R1 ), 6. 9-189. 7 ng ( ginsenoside Rg1 ), 6. 8-171. 4 ng (ginsenoside Re), 9. 4-156. 1 ng (ginsenoside Rb1), 7. 5-180. 5 ng (ginsenoside Rd). The recovery was between 95%-105%. The results show that the pCEC-μELSD system can be used for the determination of the content of active ingredients in medicines. The construction ofμELSD provides an innovative detection means for capillary liquid chromatography, pCEC and capillary electrophoresis.关键词
微流蒸发光散射检测器/加压毛细管电色谱/血塞通/三七皂苷/人参皂苷Key words
Pressurized capillary electrochromatography/Microflow evaporative light scattering detector/Xuesaitong ( lyophilization)/Notoginsenoside/Ginsenoside引用本文复制引用
高红秀,周文莉,王玉红,李鹏,谷雪,王彦,李静,阎超..加压毛细管电色谱-微流蒸发光散射检测器联用系统的构建及其应用[J].分析化学,2014,(5):766-772,7.基金项目
本文系国家重大科学仪器设备开发专项项目(No.2011YQ150072)、国家自然科学基金项目(Nos.21175092,.21105064)、上海市自然科学基金项目(No.12ZR1413600)和上海市引进技术的吸收与创新计划项目(No. JJ-YJCX-03-10-2586)资助 （No.2011YQ150072）
