法医学杂志Issue(5)：356-359,4.DOI:10.3969/j.issn.1004-5619.2013.05.010
硅藻硝酸消化法与浮游生物16S rDNA PCR法在溺死鉴定中的比较
Comparative Analysis between Diatom Nitric Acid Digestion Method and Plankton 16S rDNA PCR Method
摘要
Abstract
Objective To com pare and explore the application value of diatom nitric acid digestion method and plankton 16S rDNA PCR method for drow ning identification. Methods Forty drow ning cases from 2010 to 2011 were collected from Department of Forensic Medicine of Wenzhou Medical University. Sam ples including lung, kidney, liver and field water fromeach case were tested with diatom nitric acid digestion method and plankton 16S rDNAPCR method, respectively. The Diatom nitric acid digestion method and plankton 16S rDNAPCR method required 20 gand 2g of each organ,and 15 mL and 1.5 mL of field water, respectively. The inspection time and detection rate were com pared between the two methods. Results Diatom nitric acid digestion method m ainly detected two species of diatom s, Centriae and Pennatae, while plankton 16S rDNA PCR method am plified a length of 162 bp band. The average inspection time of each case of the Diatom nitric acid digestion method was (95.30±2.78) min less than (325.33±14.18)min of plankton 16S rDNA PCR method (P<0.05).The detection rates of two methods for field water and lung were both 100% . For liver and kidney, the detection rate of plankton 16S rDNA PCR method was both 80% , higher than 40% and 30% of diatom nitric acid digestion method (P<0.05), respectively. Conclusion The laboratory testing method needs to be appropriately selected according to the specific circum stances in the forensic appraisal of drow ning. Com pared with diatom nitric acid digestion method, plankton 16S rDNA PCR method has practice values with such advantages as less quantity of sam ples, huge inform ation and high specificity.关键词
法医病理学/溺水/16S rDNA/聚合酶链反应/硝酸消化法/浮游生物/硅藻类Key words
forensic pathology/drow ning/16S rD N A/polym erase chain reaction/nitric acid digestion m ethod/plankton/diatom s分类
社会科学引用本文复制引用
韩军鸽,王程宝,李兴彪,范琰琰,冯相平..硅藻硝酸消化法与浮游生物16S rDNA PCR法在溺死鉴定中的比较[J].法医学杂志,2013,(5):356-359,4.基金项目
浙江省教育厅科研资助项目 （）
