广东工业大学学报Issue(1):136-140,5.DOI:10.3969/j.issn.1007-7162.2014.01.027
诱导前后地鳖血淋巴抗菌活性的研究
A Study of Antibacterial Activity of Haemolymph from Eupolyphage by Inducement
摘要
Abstract
Eupolyphage sinensis Walker adults (female) were injected with 5 μL E.coli、Staphylococcu-saureus and Bacillus subtilis respectively via intravitreal injection .The antibacteial activity of haemolymph extracted from induced Eupolyphage for 24, 48 and 72 h respectively to the three bacterial was measured with the filter paper method and compared with the uninduced Eupolyphage as the negative control .The strongest antibacterial activity of haemolymph induced by Staphylococcus aureus for 48h was chosen and then the influence on haemolymph of such factors as the increased sample , pH, temperature and heating time wereanalyzed .The results show that the antibacterial activity of hemolymph enhances with the in-creasing amountof the sample; The optimal pH value is 5.0; the antibacterial activity of hemolymph is sensitive to heat ,and thereis no action at the temperatures of 80 ℃or 100 ℃within a short period .关键词
地鳖/血淋巴/诱导/抗菌Key words
Eupolyphage/haemolymph/inducement/antibacterial activity分类
化学化工引用本文复制引用
代春迎,谭竹钧,黎子蔚..诱导前后地鳖血淋巴抗菌活性的研究[J].广东工业大学学报,2014,(1):136-140,5.基金项目
国家自然科学基金资助项目(30772739);广东工业大学211工程培育项目 ()