高等学校化学学报Issue(3)：652-659,8.DOI:10.7503/cjcu20130708
葡聚糖分子量和接枝度对阿霉素/白蛋白-葡聚糖纳米粒子体外抗肿瘤效果的影响
In Vitro Anticancer Effects of Doxorubicin Loaded BSA-dextran Nanoparticles with Different Molecular Weights of Dextran and Different Dextran Conjugation Degrees
摘要
Abstract
Bovine Sercurn albumin-dextran( BSA-dextran) conjugates were prepared with different molecular weights of dextran and different molar ratios of BSA to dextran via Maillard reaction. Doxorubicin loaded BSA-dextran nanoparticles were fabricated by changing the pH and then temperature of the mixture. The nanoparti-cles with 5í103 and 10í103 dextran have a size about 60 nm, and the nanoparticles with 62í103 dextran have a size about 200 nm. The doxorubicin loading efficiency is in the range of 81%-98%, and the loading amount is 7.4%-16.9%. In vitro cell viability investigation confirms the excellent biocompatibility of BSA-dextran conjugates. Compared with free doxorubicin, the nanoparticles can enhance the cellular internalization of the loaded doxorubicin and they show lower anticancer activity at lower doxorubicin concentrations because of the slower release of the loaded doxorubicin. In comparison with the nanoparticles with 62í103 and 10í103 dextran, the nanoparticles with 5í103 dextran and higher dextran conjugation degree show better cellular inter-nalization and better anticancer activity in vitro due to their smaller size, denser dextran brush surface, certain free doxorubicin concentration, and faster release rate of the loaded doxorubicin.关键词
葡聚糖/牛血清白蛋白/药物释放/纳米粒子/抗肿瘤活性/阿霉素Key words
Dextran/Bovine Sercurn albumin ( BSA )/Drug delivery/Nanoparticles/Anticancer activity/Doxorubicin分类
化学化工引用本文复制引用
郝和群,姚萍..葡聚糖分子量和接枝度对阿霉素/白蛋白-葡聚糖纳米粒子体外抗肿瘤效果的影响[J].高等学校化学学报,2014,(3):652-659,8.基金项目
国家重点基础研究发展计划(批准号:2011CB932503)和国家自然科学基金(批准号:21274026)资助.@@@@ Supported by the State Key Development Program for Basic Research of China(No.2011CB932503) and the National Natural Science Foun-dation of China(No.21274026) （批准号:2011CB932503）
