高等学校化学学报Issue(4)：724-729,6.DOI:10.7503/cjcu20130718
新型核素64 Cu标记D-脱氧葡萄糖及其小动物正电子发射断层扫描显像
Radio-labeling and Micro-PET Study of 64 Cu Labeled D-Glucose for Tumor Imaging
摘要
Abstract
2-(4-Isothiocyanatobenzyl)-1,4,7,10-tetraazacyclo-dodecane-1,4,7,10-tetraacetic acid-D-glucose ( DOTA-DG) was synthesized and radiolabeled with 64 Cu. The DOTA-DG dissolved in sodium acetate buffer (pH=5. 5) was added to the eluent of64Cu. The solution was stirred and placed at 25℃ for 30 min. The la-beling efficiency and radiochemical purity of 64 Cu-DOTA-DG were all over 95%, as determined by radio-HPLC. The in vitro stability of the conjugates was analyzed in 0.1 mol/L PBS and 0.1 mol/L NaAc and it showed excellent in vitro stability. 14. 8 MBq of64 Cu-DOTA-DG was injected to Hep-G2 xenograft models via tale vein. Positron emission tomography images were taken 1 and 3 h after the injection. These images sugges-ted that there was high tumor uptake of 64 Cu-DOTA-DG. The results suggested that 64 Cu-DOTA-DG could provide images much clearer than the DG labeled with 99m Tc, 111 In and 68 Ga, and observation of the metabo-lism of DG much longer. These preliminary data show that 64 Cu-DOTA-DG appears to be useful in PET ima-ging.关键词
2-氨基-2-脱氧-D-葡萄糖/铜-64/正电子发射断层显像/肿瘤Key words
2-Amino-deoxy-D-glucose/64 Cu/Positron emission tomography( PET)/Tumor分类
化学化工引用本文复制引用
洪业,朱华,胡骥,林新峰,卿晶,杨志..新型核素64 Cu标记D-脱氧葡萄糖及其小动物正电子发射断层扫描显像[J].高等学校化学学报,2014,(4):724-729,6.基金项目
国家自然科学基金(批准号:81071198,81172082,81371592)和北京市自然科学基金(No.7132040)资助.@@@@Supported by the National Natural Science Foundation of China(Nos.81071198,81172082,81371592) and the Beijing Municipal Natural Science Foundation, China(No.7132040) （批准号:81071198,81172082,81371592）
