广东医学Issue(6)：805-808,4.
脂肪来源间充质干细胞移植对大鼠全层皮肤缺损的修复作用
The effects of adipose-derived stem cells transplantation in full thickness cutaneous deficiency repairing in rats
孙传伟 1陈华德 1卞徽宁 1郑少逸 1刘族安 1赖文1
作者信息
- 1. 广东省人民医院 广东省医学科学院 烧伤科 广州510080
- 折叠
摘要
Abstract
Objective To investigate the effects of adipose -derived stem cells ( ADSCs ) transplantation in full thickness cutaneous deficiency repairing in rats .Methods Full thickness cuntaneous deficiency models were constructed in 25 SD rats and randomly divided into control group , collagen membrane group ( negative control ) and ADSCs-collagen membrane group;in which allogeneic skin graft ( ASG) alone, ASG plus collagen membrane , and ASG plus ADSCs-col-lagen membrane were given for wound covering , respectively.The level of wound healing was observed .Tensile test, HE staining and in situ hybridization were carried out to assess the tensile strength , histological changes and ADSCs′expres-sion in new skin , respectively .Results Animal experiment showed that ADSCs and collagen membrane transplantations were safe.The wound healing rate of ADSCs -collagen membrane group [(94.24 ±3.13)%] was significantly higher than those of control group [(84.57 ±2.42)%] and collagen membrane group [(84.72 ±9.70)%].There was no sig-nificant difference was revealed in the tensile strength of new skin among the three groups ( P >0.05 ) .HE staining showed that there were neogenetic skin appendages in experimental group rather than controls .According to in situ hybrid-ization, SRY DNA was positive in the new skin of ADSCs -collagen membrane group .Conclusion ADSCs transplanta-tion can promote the full thickness cutaneous deficiency repairing in rats .关键词
脂肪来源间充质干细胞/全层皮肤缺损/创面愈合Key words
adipose-derived stem cells/full thickness cutaneous deficiency/wound healing引用本文复制引用
孙传伟,陈华德,卞徽宁,郑少逸,刘族安,赖文..脂肪来源间充质干细胞移植对大鼠全层皮肤缺损的修复作用[J].广东医学,2014,(6):805-808,4.
