干旱地区农业研究Issue(5)：110-116,206,8.
芸芥自交不亲和等位基因及其表达初步研究
Study on Salleles and their expression in self-incompatible lines of Eruca Sativa Mill.
摘要
Abstract
In the process of breeding research by ways of self-incompatible lines in E . Sativa Mill .,there is often a phenomenon that the seed setting percentage from the cross of two parents is high in bud stage ,but the number of seeds is low in late stage owing to the natural segregation .By using the method of complete diallel crossing ,the purity of S al-leles in 10 self-incompatible lines of E . Sativa Mill .was studied ,and the expression tissue of self-incompatible genes was analyzed with DDRT -PCR technique .The results showed that the self-compatible index of Yunjie 1 ,Yunjie 3 , Yunjie 5 ,Yunjie 6 ,Yunjie 8 ,Yunjie 9 and Yunjie 13 was below 1 after selfing or outcrossing among these 7 self-incom-patible lines ,that is to say ,their self-incompatibility was stable .However ,the self-incompatibility of Yunjie 2 ,Yunjie 4 and Yunjie 10 was not stable .DDRT-PCR analysis showed that the self-incompatible genes of E .Sativa Mill .didn’ t belong to constitutive expression ,but to specific tissue expression .Therefore ,when creating F1 hybrids by ways of self-incompatible lines ,it is necessary for us to determinate the purity of S alleles and their genotypes besides agronomic traits and self-incompatible stability .关键词
芸芥/自交不亲和系/S等位基因/基因表达/DDRT-PCRKey words
Eruca sativa Mill./self-incompatible lines/Salleles/gene expression/DDRT-PCR分类
农业科技引用本文复制引用
范惠玲,王文倩,李彦龙,白生文..芸芥自交不亲和等位基因及其表达初步研究[J].干旱地区农业研究,2013,(5):110-116,206,8.基金项目
河西学院大学生科技创新项目（2011-101）；河西学院青年教师科研基金项目 （）
