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深绿木霉发酵液对黑麦草促生作用及生理生化特性的影响

张树武 徐秉良 程玲娟

干旱地区农业研究Issue(2):157-162,6.
干旱地区农业研究Issue(2):157-162,6.

深绿木霉发酵液对黑麦草促生作用及生理生化特性的影响

Effects of fermentation medium of Trichoderma aureoviride on growth and physiological characteristics of ryegrass

张树武 1徐秉良 2程玲娟3

作者信息

  • 1. 甘肃农业大学草业学院,甘肃 兰州 730070
  • 2. 草业生态系统教育部重点实验室,甘肃 兰州 730070
  • 3. 甘肃省草业工程实验室,甘肃 兰州 730070
  • 折叠

摘要

Abstract

Effects of different dilutions of the fermentation medium of Trichoderma aureoviride on growth and physio-logical characteristics of ryegrass were studied by seed germination and seedling pot experiment in vitro and pot experi -ments .Results showed that different dilutions of the fermentation medium of T .aureoviride can significantly increase the rates of seed germination ,germination index and vigor index ,especially for the dilutions of 100 times by which seed ger-mination rates ,germination index and vigor index were increased by 96 .55% ,37 .41 and 7 .83 ,respectively .Pot exper-iment results showed that different dilutions of the fermentation medium of T . aureoviride had obvious effects on the growth of ryegrass seedlings ,and can significantly increase the content of chlorophyll ,protein ,and defensive enzymes . Especially the dilutions of 100 times can increase ryegrass root length ,plant height ,fresh weight and dry weight by 16 .95% 、13 .33% 、40 .57% 、73 .68% ,and 36 .36% ,respectively .The content of chlorophyll and soluble protein were increased by 84 .09% and 75 .50% .Polyphenol oxidase ,peroxidase and phenylalanine enzyme activity were significantly higher than control after treatment with the dilutions of 100 times of T . aureoviride .Maximum activities of POD ,PPO and PAL were observed on 3 and 4 days after inoculation .Therefore ,the results suggested that the fermentation medium of T . aureoviride has substantial effects on growth and physiological characteristics of ryegrass .

关键词

深绿木霉/促生作用/黑麦草/生理生化特性

Key words

Trichoderma aureoviride/growth promoting/ryegrass/physiological characteristics

分类

农业科技

引用本文复制引用

张树武,徐秉良,程玲娟..深绿木霉发酵液对黑麦草促生作用及生理生化特性的影响[J].干旱地区农业研究,2014,(2):157-162,6.

基金项目

草业生态系统教育部省部共建重点实验室项目(CY - GG -2006-013);甘肃省农牧厅生物技术专项(GNSW -2009-04);甘肃省教育厅项目 ()

干旱地区农业研究

OACSCDCSTPCD

1000-7601

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