国际医药卫生导报2014，Vol.20Issue(7)：889-892,4.DOI:10.3760/cma.j.issn.1007-1245.2014.07.001
HPLC法同时测定一清软胶囊中8种有效成分的含量
Simultaneous quantitative determination of eight active components in Yiqing Soft capsule by HPLC
摘要
Abstract
Objective To establish a method for simultaneous quantitative determination of eight active components in Yiqing Soft capsule.Methods The separation was performed on Xtimate-C 18 chromatographic column with mobile phase consisted of water (containing 0.2％ phosphoric acid)-acetonitrile (gradient elution) at a flow rate of 0.6 ml/min.Detecting wavelength was set at 280 nm and the column temperature was set at 30℃.Results The calibration curve of berberine,coptisine-hydrochloride,baicalein,aloe-emodin,rhein,emodin,chrysophanol and physcion showed good linear regression in test ranges (r ＞ 0.9995); and the overall recoveries were in the range of 97％-103％ (n=3).The overall RSD of precision test were less than 2％.Conclusion The extracting method is convenient and credible,and the quantitative method is accurate and repeatable,and it can be used in simultaneous quantitative determination of eight active components in Yiqing soft capsule.关键词
一清软胶囊/高效液相色谱法/含量测定Key words
Yiqing soft capsule/ HPLC/ Content determination引用本文复制引用
班莉,李开莹,罗钊,杜清涛,黎行山,郭丽冰..HPLC法同时测定一清软胶囊中8种有效成分的含量[J].国际医药卫生导报,2014,20(7):889-892,4.基金项目
国家自然科学基金项目(81073045) （81073045）
