桂林理工大学学报Issue(4)：688-693,6.DOI:10.3969/j.issn.1674-9057.2013.04.019
枸杞幼苗对钒胁迫的生理响应和积累特性
Bioaccumulation and Physiological Responses of Lycium barbarum Seedlings to Vanadium Stress
摘要
Abstract
Lycium barbarum seedlings were subjected to varying concentration (0, 25, 50, 100, 200 mg· kg -1) of vanadium (V) for 40 days to investigate the accumulation of V in Lycium seedling, the effects of V on cell membrane permeability, the antioxidant enzyme activities, contents of the chlorophyll, malondialdehyde (MDA), proline (Pro) and soluble protein in leaves of Lycium barbarum seedlings.The results showed that the accumulation of V in Lycium barbarum seedlings was as following: root>leaf>stem.The activities of per-oxidase (POD) and catalase ( CAT), the contents of chlorophyll and protein first increased and then de-creased with the increasing V supply levels.When V concentration was less than 100 mg· kg -1, the activities of POD and CAT, the contents of chlorophyll and protein increased by 227.9%, 76.2%, 40.2% and 117.4%, respectively when compared with the control. They decreased by 5.1%, 5.8%, 22.8% and 36.7%, respectively when V concentration was higher than 100 mg· kg -1.With different V stress, MDA content, cell membrane permeability and proline content increased 13.3%-220.7%, 12.4%-76.9% and 69.7%-276.3% respectively.The investigation shows enhanced activity of chlorophyll and antioxidant en-zyme, and synthesization of soluble protein when V stress level was less than 100 mg· kg-1.While the struc-ture of chlorophyll was damaged, membrane lipid peroxidization, cell membrane permeability and the content of free proline increased, synthesization of antioxidant enzymes and soluble protein was restrained when V stress level was higher than 100 mg· kg-1 .关键词
钒胁迫/枸杞幼苗/生理响应/积累特性Key words
vanadium stress/Lycium barbarum seedlings/physiological responses/bioaccumulation分类
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侯明,陈如,黄艳娇..枸杞幼苗对钒胁迫的生理响应和积累特性[J].桂林理工大学学报,2013,(4):688-693,6.基金项目
国家自然科学基金项目（41161076）；广西自然科学基金项目（2011GXNSFA018045）；广西环境工程与保护评价重点实验室研究基金项目 （）
