感染、炎症、修复Issue(1)：18-22,5.DOI:10.3969/j.issn.1672-8521.2014.01.004
骨髓间充质干细胞对糖尿病小鼠创面早期炎症细胞的影响及机制初探
Study on effect of bone marrow mesenchymal stem cells on acute inflammatory cell infiltration in a wound and its mechanism
摘要
Abstract
Objective:To study the effect of bone marrow mesenchymal stem cells (BMSCs)on infiltration of neutrophils (PMNs)and macrophages (MΦ),mRNA expression of macrophage inflammatory protein-2 (MIP-2),and monocyte chemo-tactic protein-1 (MCP-1)in a genetical modification-induced spontaneous diabetic murine model. Methods:Forty C57BLKS/Nj u spontaneous mutation resulted diabetic mice (db/db mice)were randomly divided into two groups:BMSCs group and control group (n=20).Full-thickness skin defects of 8 mm×8 mm were created on the dorsum of each mouse. Mice in BM-SCs group were treated with 1×106 BMSCs via subcutaneous injection around the defect after injury,but in control group, PBS was injected instead of BMSCs. Wound healing rate was assayed on postwound day 1,3,5,7,10 and 14 with image pro-cessing software IPP6.0. The infiltration of PMNs (postwound day 1,3,5,7 and 10)and MΦ(postwound day 3,5,7,10 and 14)was observed by immunohistochemical method and optical density value detection. The relative quantitative mRNA expression of MIP-2 and MCP-1 of the wound tissues on postwound day 1,3,5 and 7 was determined by qRT-PCR relative quantitative analysis.Results:The wound healing time was (11.67±0.58)days and (16.33±0.58)days respectively in BM-SCs group and PBS group (P<0.05).Infiltration of PMNs and MΦwas more marked in BMSCs group(P<0.01),with earlier peaking than in PBS group. mRNA expression of MIP-2 and MCP-1 in BMSCs group was statistically elevated in postwound day 1 and day 3,respectively (P<0.05 ),which were 30.91 and 96.88 times of those in the control group.Conclusions:BMSCs might enhance infiltration of PMNs and MΦby promoting the expression of MIP-2 and MCP-1 in wound,and they might also contribute to accelerate healing process of diabetic wound when treated with BMSCs.关键词
骨髓间充质干细胞/糖尿病创面/炎症细胞/趋化因子Key words
Bone marrow stem cells/Diabetes wound/Inflammatory cells/Chemokine引用本文复制引用
邹吉平,黄沙,宣敏,程飚..骨髓间充质干细胞对糖尿病小鼠创面早期炎症细胞的影响及机制初探[J].感染、炎症、修复,2014,(1):18-22,5.基金项目
国家自然科学基金资助项目(81272105,81171812) （81272105,81171812）
博士后科学基金资助项目(21003777) （21003777）
