中国妇幼健康研究Issue(4)：492-494,3.DOI:10.3969/j.issn.1673-5293.2013.04.013
胚胎中HBV mRNA与母婴垂直传播关系的研究
Relation between HBV mRNA in the embryo of HBV patients and vertical transmission
摘要
Abstract
Objective To confirm whether vertical transmission of hepatitis B virus ( HBV) can occur via infected ovum by detecting HBV mRNA in the test-tube embryos of mothers with chronic HBV .Methods Specimens were discarded test-tube embryos from mothers with chronic HBV infection who had received in vitro fertilization treatment .Single-cell reverse transcriptase-polymerase chain reaction ( RT-PCR) was used to detect HBV mRNA in the embryos .Results HBV mRNA was detected in the cleavage embryos of 5 cases with chronic HBV infection, with a detection rate of 13.2%(5/38), including 3 embryos of the 5 discarded ones detected with positive signal .Maternal HBeAg was positive, and quantitative HBVDNA was 4.7 ×105IU/mL.Positive signal was detected in 2 of another 3 discarded embryos. Maternal HBeAg was negative with quantitative HBVDNA≤103 IU/mL.Conclusion HBV can be transferred to embryo via ovum , and vertical transmission is confirmed .Meanwhile , HBV is duplicated in embryo .关键词
乙型肝炎病毒/胚胎/乙型肝炎病毒信使RNA/母婴垂直传播Key words
HBV/embryo/HBV mRNA/vertical tranmission分类
医药卫生引用本文复制引用
刘昳,张健,宗时春,叶峰,师娟子,金燕,蔺淑梅,孔颖,张曦,杨雪亮..胚胎中HBV mRNA与母婴垂直传播关系的研究[J].中国妇幼健康研究,2013,(4):492-494,3.基金项目
国家自然科学基金资助项目 （）
