中国妇幼健康研究Issue(5)：672-673,694,3.DOI:10.3969/j.issn.1673-5293.2013.05.015
子宫腺肌病小鼠模型建立及对生育力的影响
The model of uterine adenomyosis in mice induced by tamoxifen and the effect on fertility
夏丽群 1朱海斌 1夏雅仙1
作者信息
- 1. 浙江大学医学院附属第一医院妇科，浙江杭州 310003
- 折叠
摘要
Abstract
Objective To establish adenomyosis ( AM) model of mice by oral administration of tamoxifen to Institute of Cancer Research ( ICR) mice, and to study the fertility of the AM model .Methods Twenty-four newly born ICR mice with AM were induced by oral administration of tamoxifen from day 2 after birth.The histomorphology of uterus on day 42 and 90 was observed .The models of mice with AM were mated to normal male mice on day 90, and the implantation of embryos in uterus on 9th pregnant day was observed .Results There was infiltration of stroma in the endometrium and disordered development in tunica muscularis in the uterus of model mice on day 42. There were typical manifestations of AM in model mice on day 90, endometrium infiltrating muscular layer but not reaching the subserosa . In vivo experiment, a significantly declining of fertility in AM model mice was found when they were compared with control mice (Fisher’ s value was 0.000, P<0.01).Conclusion Oral administration of tamoxifen can induce the mouse model of AM successfully .The fertility of AM mice declines significantly , and they can be taken as ideal animal models to study the disease of AM in reproductive area .关键词
子宫腺肌病/小鼠/他莫昔芬/生育力Key words
adenomyosis ( AM)/mouse/tamoxifen/fertility分类
医药卫生引用本文复制引用
夏丽群,朱海斌,夏雅仙..子宫腺肌病小鼠模型建立及对生育力的影响[J].中国妇幼健康研究,2013,(5):672-673,694,3.
