摘要
Abstract
Objective To explore the protective effects of recombinant human erythropoietin ( rhEPO) on hypoxic-ischemic brain damage in neonatal rats .Methods Fifty-seven 7-day-old Sprague-Dawley rats were randomly assigned to 3 groups: sham-operated group ( control group), hypoxic-ischemic brain damage model (HIBD group) and rhEPO-intervention group.Immediately after reparation of HIBD model , the rhEPO-intervention group was injected 3 000U/kg of rhEPO into the abdominal cavity , and the HIBD group was injected the same dose of normal saline .After 24 hours histological changes in brain tissue were observed with electron microscope .Apoptosis of damage neuron was measured by flow cytometry (FCM).Four separate reflexes were evaluated at 7day.Results Compared with the sham-operated group, the rats in HIBD group showed obvious histo-pathological damage in left brain.The apoptosis of neuron increased (28.30 ±4.80 vs 47.14 ± 6.97, P<0.05) and the survived cells decreased (72.42 ±7.93 vs 48.33 ±8.39, P<0.05).Compared with the HIBD group, the neuronal apoptosis decreased (47.14 ±6.97 vs 36.88 ±9.43,P<0.05) and survived cells increased (48.33 ±8.39 vs 60.06 ±8.32, P<0.05) in rhEPO-intervention group.The evaluation on four reflections ( suspension test, righting reflex, cliff aversion reflex and tendency reflection ) in HIBD group was worse than that in the control group , which indicated nerve function injury .Compared with the HIBD group, the righting reflex and cliff aversion reflex were shortened in rhEPO-intervention group (4.59 ±0.11 vs 2.84 ±0.13, 10.84 ± 1.26 vs 8.49 ±1.03, P<0.05).The differences between groups and among groups were all significant .Conclusion Extrinsic rhEPO can reduce pathologic injury of brain tissues in HIBD rats and plays a neuroprotective role through stabilizing cell structure , inhibiting cell apoptosis and improving short-term behavioral assessment .关键词
脑缺氧缺血/重组人促红细胞生成素/凋亡/流式细胞学分析Key words
cerebral hypoxia ischemia/recombinant human erythropoietin (rhEPO)/apoptosis/flow cytometry (FCM)分类
医药卫生
