国际口腔医学杂志Issue(2)：133-136,4.DOI:10.7518/gjkq.2014.02.003
血管内皮生长因子基因修饰骨髓间充质干细胞移植于放射治疗后组织的实验研究
Transplantation of vascular endothelial growth factor gene-modified bone mesenchymal stem cells into irradiated tissue
摘要
Abstract
Objective This study aimed to resume the ability of revascularization of the irradiated tissue by implantation of vascular endothelial growth factor(VEGF)-modified bone mesenchymal stromal cell(BMSC). Methods Each mouse was irradiated with 60Coγray on their right leg. The irradiated muscular tissues of the animal models were injected with VEGF165-modified BMSCs, and a second dose was administered after two weeks. We determined the expression of VEGF165 mRNA, VEGF165 protein, and ultrastructure of the vessels and nerves after gene therapy. Results VEGF165 mRNA and VEGF165 protein had high levels of expression in the irradiated tissue after the injection of VEGF165 -modified BMSCs. The configuration and ultrastructure of the vessels and nerves returned to normal. Conclusion VEGF165-modified BMSCs can resume the ability of the vessels and nerves of an irradiated tissue. This study provides abasis for the clinical application of VEGF165-modified BMSCs.关键词
骨髓间充质干细胞/血管内皮生长因子/放射损伤Key words
bone mesenchymal stem cell/vascular endothelial growth factor/irradiated injury分类
医药卫生引用本文复制引用
王涛,廖天安,王鸿,邓伟,于大海..血管内皮生长因子基因修饰骨髓间充质干细胞移植于放射治疗后组织的实验研究[J].国际口腔医学杂志,2014,(2):133-136,4.基金项目
海南省自然科学基金 （）
