国际病理科学与临床杂志Issue(4)：322-326,5.DOI:10.3969/j.issn.1673-2588.2013.04.010
新一代测序技术及其在肿瘤研究中的应用
Next generation sequencing technology and its application in cancer research
摘要
Abstract
The next generation sequencing (NGS), also known as high-throughput sequencing technology, is a landmark of revolution in the history for DNA sequencing. NGS have adopted a new sequencing strategy-cyclic-array sequencing, which was divided into pyrosequencing (Roche/454), Solexa sequencing technology (Illumina), the polony sequencing technology (ABI/SOLiD), Polonator sequencing technology and HeliScope single-molecule sequencing technology. Because of its high-throughput, low cost and short time-consuming, NGS has the potential to replace traditional Sanger sequencing and to become the most important research tools in post-genomic era.Researchers could do more comprehensive and in-depth analysis with genome, transcriptome and proteome at cheaper price. NGS has broad application prospects in the field of cancer research. In addition to accomplishing the tumor genome sequencing quickly, it also provides us a fully and rapidly powerful digital tool for tumor transcriptome, epigenetic and DNA-protein interaction analysis. It is expecting to bring about revolutionary change in the areas of life science, medicine science and others.关键词
新一代测序/肿瘤/基因组/转录组/表观遗传Key words
next generation sequencing/tumor/genome/transcriptome/epigenetic引用本文复制引用
肖岚(综述)..新一代测序技术及其在肿瘤研究中的应用[J].国际病理科学与临床杂志,2013,(4):322-326,5.基金项目
国家自然科学基金(81101541)；湖南省自然科学基金(11JJ4072)；高等学校博士学科点专项科研基金(20110162120038)。This work was supported by the National Natural Science Foundation of China (81101541), Natural Science Foundation of Hunan Province (11JJ4072), and Specialized Research Fund for the Doctoral Program of Higher Education (20110162120038), P.R.China （81101541）
