神经损伤与功能重建Issue(6)：414-417,4.DOI:10.3870/sjsscj.2013.06.007
玻璃体内单剂量注射睫状神经营养因子对金黄地鼠视神经再生的影响
Single Intravitreal Injection of Ciliary Neurotrophic Factor (CNTF) Promotes Optic Nerve Re-growth in Golden Hamsters
吕强 1赵发国 1张卫清 1张英谦 1石进1
作者信息
- 1. 北京空军总医院神经内科北京 100142
- 折叠
摘要
Abstract
Objective:To observe the effect of single intraviteal injection of CNTF on the axonal regeneration of retinal ganglion cells in the autologous sciatic nerve transplants in golden hamsters. Methods: Twenty golden hamsters were randomly divided into normal control group (n=2), control group (A group, n=6), CNTF 2 μg group (B group, n=6) and CNTF 1μg (C group, n=6). In all the hamsters, a segment of autologous sciatic nerve was transplanted to the orbital end of the transected optic nerve. The animals in the groups A, B, and C were immediately intravitreally injected with 2 μL of normal saline, 2 μL CNTF solution containing 2μg CNTF, and 2μL CNTF solution containing 1μg CNTF respectively. The regenerating retinal ganglion cells (RGCs) was visualized by flouro-gold and the labeled RGCs were counted. Results: The number of regeneration of retinal ganglion cells of the groups A, B, and C were 4932, 8596, and 7338 respectively. The number of labeled ganglion cells in the groups B and C were significantly higher than that of the group A ( <0.05). There was no statistical difference between B and C groups. Conclusion:Single intravitreal injection of CNTF can markedly increase the axonal regrowth of RGCs in sciatic nerve transplants in hamsters.关键词
睫状神经营养因子/视网膜节细胞/视神经/玻璃体内注射/神经再生/金黄地鼠Key words
ciliary neurotrophic factor/retinal ganglion cells/optic nerve/intravitreal injection/neuroregenera-tion/golden hamster分类
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吕强,赵发国,张卫清,张英谦,石进..玻璃体内单剂量注射睫状神经营养因子对金黄地鼠视神经再生的影响[J].神经损伤与功能重建,2013,(6):414-417,4.
