神经损伤与功能重建Issue(2)：87-91,5.DOI:10.3870/sjsscj.2014.02.001
离体神经干细胞氧糖剥夺模型的建立及改变培养条件对神经干细胞的影响分析
Effects of Oxygen and/or Glucose Deprivation on Neural Stem Cells Culture
王倩 1熊永洁 2张苏明 3甘莉 3李珍1
作者信息
- 1. 华中科技大学同济医学院第二临床学院
- 2. 漯河市中心医院河南 漯河 462000
- 3. 华中科技大学同济医学院附属同济医院神经内科武汉 430030
- 折叠
摘要
Abstract
ObjectiveTo establish a oxygen-glucose deprivation (OGD) model of neural stem cells (NSCs) and to investigate the effects of oxygen deprivation, glucose deprivation, OGD and high glucose on the survival and proliferation of NSCs culture respectively. Methods:The NSCs were separated from cortices of fetal mice and i-dentified. For the establishment of OGD, the NSCs were cultured in glucose-free DMEM and placed in a modular anaerobic chamber. The NSCs incubated in a normal condition were used as a control group. Both the cells in the control and OGD groups were incubated for different time durations (2 h, 4 h, 6 h, 8 h, 10 h). MTT assay was used to detect the effects of OGD on the survival and proliferation of NSCs. Besides, the NSCs were randomly as-signed into the control 8h group, oxygen deprivation 8 h group, glucose deprivation 8 h group, OGD 8 h group and high glucose 8 h group, and the cell viabilities were measured with MTT assay. Results:Compared to the nor-mal condition, the cell viabilities of OGD group were decreased at the time points of 8 h and 10 h significantly ( <0.05). The cell viabilities of oxygen deprivation 8h group, glucose deprivation 8h group and high glucose 8 h group were decreased when compared to control 8h group(all <0.05). There was no significant difference among glucose deprivation 8 h group, OGD 8 h group and high glucose 8 h group. Conclusion:The growth of NSCs was decreased significantly with OGD for 8 h or longer. In the model of OGD, low glucose concentrations might be the major detrimental factor for NSCs and the glucose concentration play an important role in the survival and proliferation of NSCs.关键词
神经干细胞/细胞活性/氧糖剥夺/模型Key words
neural stem cells/cell viability/oxygen glucose deprivation/model分类
医药卫生引用本文复制引用
王倩,熊永洁,张苏明,甘莉,李珍..离体神经干细胞氧糖剥夺模型的建立及改变培养条件对神经干细胞的影响分析[J].神经损伤与功能重建,2014,(2):87-91,5.
