神经损伤与功能重建Issue(2)：92-94,3.DOI:10.3870/sjsscj.2014.02.002
激光共聚焦显微镜和免疫组化染色对比观察KCNQ1在小鼠耳蜗血管纹的表达
Expression and Subcellular Localization of KCNQ1 in Stria Vascularis of Mice Cochlea by Laser Scanning Confocal Microscopy and Immunohistochemistry Staining
摘要
Abstract
ObjectiveTo investigate the expression and distribution of KCNQ1 in stria vascularis of murine cochlea with laser scanning confocal microscopy (LSCM) and immunohistochemistry staining technique. Methods: With LSCM and immunohistochemistry staining, the location of KCNQ1 in cochlea of the CBA/CaJ and KCNQ1 -/ -mice were observed. Results: Immunohistochemical staining showed that the expression of KCNQ1 protein was found at the apical membrane wall of marginal cells in stria vascularis of CBA/CaJ mice. There was no KCNQ1 protein expression in marginal cells of KCNQ1 -/-mice. The fluorescence of KCNQl protein was mainly expressed in the cell membrane of marginal cells of CBA/CaJ mice with LSCM. Conclusion:The expression and distribution of KCNQ1 protein in marginal cells of stria vascularis could be observed with LSCM.关键词
KCNQ1/基因敲除小鼠/边缘细胞/激光共聚焦显微镜Key words
KCNQ1/gene knockout mice/marginal cell/lascer scanning confocal microscopy分类
医药卫生引用本文复制引用
吴振恭,褚汉启,熊俊,陈金川,刘云,陈金,周良强,熊浩..激光共聚焦显微镜和免疫组化染色对比观察KCNQ1在小鼠耳蜗血管纹的表达[J].神经损伤与功能重建,2014,(2):92-94,3.基金项目
国家自然科学基金（No.81271078）福建省泉州市卫生局科研课题 （）
