神经损伤与功能重建Issue(2)：105-107,118,4.DOI:10.3870/sjsscj.2014.02.005
急性脑出血大鼠肠屏障功能的变化
Changes of Intestinal Barrier Function of Rats with Acute Cerebral Hemorrhage
张继龙 1武国艳 1汪连珍 1李立为 1王智超1
作者信息
摘要
Abstract
ObjectiveTo observe the changes of intestinal barrier function of rats with acute cerebral hemorrhage. Methods:Sixty Wistar rats were randomly divided into cerebral hemorrhage group and control group with 30 rats in each group. The cerebral hemorrhage model was established by stereotactic infusing 50 μL autologous caudate artery blood. The control group were only infused with equal normal saline. The concentra-tion of plasma diamine oxidase (DAO) and activity of D-lactate (D-lac) in plasma were measured before operation and 0.5, 3, 6, 12, 24 h after operation. The concentration of plasma Lipopolysaccharide (LPS) was measured before operation and 12, 24 h after operation. The jejunums (1 cm) of the both groups were taken at 24 h after operation, and light microscopic examination was performed for morphological measurement of intestinal epithelial cells. Results:Compared with those in the control group, the DAO and LPS concentrations at 12, 24 h and the D-lac levels at 6,12, 24 h after operation were significantly increased in the cerebral hemorrhage group ( <0.05 or 0.01). Under the light microscope, intestinal mucosal injuries were observed in the cerebral hemorrhage group whereas no obvious changes occurred in the control group . Conclusion:Intestinal barrier function was damaged in the early stage of cerebral hemorrhage.关键词
急性脑出血/肠屏障功能/二胺氧化酶/D-乳酸/内毒素Key words
acute cerebral hemorrhage/intestinal barrier function/diamine oxidase/D-lactate/Lipopolysaccha-ride分类
医药卫生引用本文复制引用
张继龙,武国艳,汪连珍,李立为,王智超..急性脑出血大鼠肠屏障功能的变化[J].神经损伤与功能重建,2014,(2):105-107,118,4.
