高校化学工程学报Issue(5)：779-784,6.DOI:10.3969/j.issn.1003-9015.2013.05.009
纤维素酶法提取竹叶黄酮的传质动力学
Mass Transfer Kinetics of Flavonoids Extraction from Baantant Leaves with Cellulase-Assisted Aqueous Extractant
摘要
Abstract
In this study, cellulase-assisted aqueous extractant (CAE) and aqueous extractant (AE) were adopted separatively for the extraction of the total flavonoid compounds from bamboo leaves. The influence of temperature, raw material size, rotation speed of stirrer and cellulase dosage on the mass transfer rates of the flavonoid compounds was examined. In addition, the micro-structural changes of bamboo leaf cells after the CAE or AE process were observed by scanning electron microscopy (SEM). Results show that the Fick’s second law fits well with the experimental data and the extraction rate is controlled mainly by the inner diffusion process. The presence of cellulase has nearly no influence on the mass transfer mechanism, and can only damage the cell walls and reduce the resistance of diffusion. The mass transfer rate and apparent diffusion constant D′ are increased accordingly. The relationship of D′with the extraction temperature and the bamboo leaf size was deduced. D′ varies from 1.95×10-13 to 6.85×10-13 m2×s-1 for CAE and from 1.73×10-13 to 6.05×10-13 m2×s-1 for AE in a temperature range between 30 and 60℃. The activation energy Ea is 11.853 kJ×mol-1 for CAE, which is 9.59%lower than that for AE. The results could provide valuable theory basis for the technical design and industrial application of using CAE process to extract the flavonoid compounds from bamboo leaves.关键词
竹叶黄酮/纤维素酶辅助提取/传质动力学/有效扩散系数Key words
bamboo flavonoids/cellulase-assisted aqueous extraction/mass transfer kinetics/apparent diffusion constant分类
化学化工引用本文复制引用
魏凤玉,陈玮,方菊,宝呼和..纤维素酶法提取竹叶黄酮的传质动力学[J].高校化学工程学报,2013,(5):779-784,6.基金项目
安徽高校省级自然科学研究重点项目(KJ2009a002)；国家大学生创新性实验计划项目(091035947)。 （KJ2009a002）
