南方农业学报Issue(3)：352-357,6.DOI:10.3969/j.issn.2095-1191.2014.3.352
中早熟马铃薯品种（系）遗传多样性ISSR分析
Genetic diversity analysis of early and middle maturity potato varieties (strains) using ISSR markers
摘要
Abstract
[Objective] Genetic relationship and diversity of early and middle maturity potato varieties (strains) were studied using ISSR markers in order to provide references for early and middle maturity potato breeding parent selection in southern winter-cropping areas. [Method]Eight screening ISSR primers were used to assess the genetic diversity among 33 potato varieties (strains) planted in different areas of Guangxi.[Result]The results showed that 8 primers amplified 46 bands, out of which 29 bands were polymorphic, and the percentage of polymorphic loci was 63.0%. Genetic similarity coefficient of 33 potato varieties (strains) changed from 0.63045 to 1.00000, with an average of 0.80967. Cluster analysis results indicated that the potato varieties (strains) could be classified into four groups at 0.79000 similarity level. The first group included DE03-34-6, JL03-786, Zhongshu 19 and Zhongshu 18. Only Zhongshu 6 was in the second group. The third group contained Longji 1 and DE03-79-1. The rest varieties (strains) were clustered into the fourth subgroups. Varieties with close affinity (like Zhongshu 13&Zhongshu 14, Zhongshu 18&Zhongshu 19) could be clustered into close groups, which meant that potato va-rieties could be precisely clustered using ISSR markers. [Conclusion]The tested 33 potato varieties (strains) have abundant genetic diversity. Parents can be selected by genetic distances of cluster analysis, which could provide references for early and middle maturity potato breeding parent selection and heterosis utilization in southern winter-cropping areas.关键词
马铃薯/ISSR/遗传多样性/聚类分析Key words
potato/ISSR/genetic diversity/cluster analysis分类
农业科技引用本文复制引用
何海旺,李丽淑,谭冠宁,何虎翼,何新民,王晖,唐洲萍..中早熟马铃薯品种（系）遗传多样性ISSR分析[J].南方农业学报,2014,(3):352-357,6.基金项目
广西自然科学基金项目（2011GXNSFB018038）；广西科学研究与技术开发计划项目 （）
