南方农业学报Issue(3)：383-388,6.DOI:10.3969/j.issn.2095-1191.2014.3.383
马铃薯新品种桂农薯1号组培快繁研究
Tissue culture and rapid propagation of a new potato variety Guinongshu 1
摘要
Abstract
[Objective]The tissue culture and rapid propagation of a new potato variety Guinongshu 1 were studied to pro-vide technical support for industrializing production of virus-free potato seeds.[Method]The stem tip detoxification method was adopted and stem tip was used as explants. The single factor (3% NaClO and 0.1% HgCl2) and two factors (75% alcohol and 0.1%HgCl2) disinfection treatments were conducted at different sterilization times. Stem tip with the size of 0.3-0.7 mm were induced into seedlings in induction medium with different concentrations of GA3, 6-BA and combinations of 6-BA and NAA. The induced single lateral bud was cut and inoculated in MS culture medium of single factor and two factors combina-tion added with 6-BA and PP33 to proliferate. [Result]The sterilization effect of two factors of 75%alcohol+0.1%HgCl2 was better than single factor of 3%NaClO or 0.1%HgCl2, and the pollution rate declined to 21.67%. Both GA3 and 6-BA could in-duce germination of stem tip with an induced trend of increase-decrease. The induction rate was as high as 53.67%for combi-nation of 1.5 mg/L 6-BA and 0.2 mg/L NAA. At multiplication stage, the multiplication rate of combination treatment adding 0.1 mg/L PP333 and 2.5 mg/L 6-BA was as high as 6.59 times and the seedlings grew better with strong stems and leaves, and normal color. [Conclusion]For Guinongshu 1 explants, the best sterilization treatment was 75%alcohol 2 min+0.1%HgCl2 11 min and the best stem tip induction formula was MS+1.5 mg/L 6-BA+0.2 mg/L NAA. The best multiplication medium was MS+2.5 mg/L 6-BA+0.1 mg/L PP333.关键词
桂农薯1号/组织培养/茎尖/消毒/芽诱导/增殖Key words
potato/Guinongshu 1/tissue culture/stem tip/sterilization/bud induction/multiplication分类
农业科技引用本文复制引用
许娟,郑虚,韦民政,唐秀桦,闫海锋,熊军,李韦柳,覃维治..马铃薯新品种桂农薯1号组培快繁研究[J].南方农业学报,2014,(3):383-388,6.基金项目
广西科学研究与技术开发计划项目（桂科合1140010-3-1，桂科转1298009-9）；广西自然科学基金项目（2010GXNS-FC013008，2011GXNSFA018114）；国家现代农业产业技术体系广西创新团队建设专项项目 （）
