南方农业学报Issue(3)：389-394,6.DOI:10.3969/j.issn.2095-1191.2014.3.389
药用植物鸦胆子内生细菌分离及其抑菌活性研究
Isolation of endophytic bacteria from medicinal plant Brucea javanica L. Merr. and its antifungal activity
摘要
Abstract
[Objective]Endophytic bacteria with antagonistic activity were isolated from medicinal plant Brucea javanica L. Merr. to provide references for developing new biological fungicides. [Method]The endophytic bacteria were isolated by tis-sue homogenization and the strains with antifungal activity were screened by in vitro and in vivo antifungal tests. The strains with stronger inhibitory activity was identified based on morphology, physiological and biochemical characteristics, 16S rDNA sequence analysis and Biolog test. [Result]There were 43, 19, 15 and 8 isolates obtained from root, leaf, stem and fruit of Brucea javanica, respectively. Especially, the strain YGB-11 isolated from root showed good antibiosis characteristics with antibacterial rate over 80.00%to Penicillium digitatum (pers.ex Fr.)Sacc of Citrus green mold and Helminthosporium torulosum (Syd.) Ashby of banana helminthosporium leaf spot. Based on morphological, physiological and biochemical characteristics, 16S rDNA sequence analysis and Biolog test, the strain YGB-11 was identified as Bacillus amyloliquefacien. [Conclusion]With wide antimicrobial spectrum and high antifungal activities, YGB-11 has promising prospects.关键词
鸦胆子/内生细菌/分离/抑菌活性/解淀粉芽孢杆菌Key words
Brucea javanica L. Merr./endophytic bacteria/isolation/antifungal activity/Bacillus amyloliquefaciens分类
农业科技引用本文复制引用
梁子宁,赖开平,朱华,韦茂春,陈龙..药用植物鸦胆子内生细菌分离及其抑菌活性研究[J].南方农业学报,2014,(3):389-394,6.基金项目
广西自然科学基金项目（2011GXNSFF018006，2012GXNSFAA053119）；广西科技合作与交流计划项目（桂科合1298015-6）；桂林市科技攻关项目 （）
