广西医学Issue(12)：1585-1587,1590,4.DOI:10.11675/j.issn.0253-4304.2013.12.03
骨桥蛋白对胰腺癌细胞金属基质蛋白酶-9转录后调控作用的研究
Study on Effects of Osteopontin on Post-Transcriptional Regulation of MMP-9 Gene Expression in PANC-1 Cells
摘要
Abstract
Objective To investigate the effects of osteopontin on post-transcriptional regulation of matrix metalloproteinase-9(MMP-9) gene expression in PANC-1 cells.Methods Real-time PCR and luciferase reporter gene assay were used to detect the effects of osteopontin on post-transcriptional regulation of MMP-9 gene expression in PANC-1 cells.Results Osteopontin prolonged the half-life of MMP-9 mRNA and up-regulated the expression of luciferase in report gene plasmid which contained 3′UTR of MMP-9 mRNA in PANC-1 cells.Conclusion Osteopontin activates the expression of MMP-9 partly through affecting post-transcriptional regulation of MMP-9 gene expression in PANC-1 cells, whose primary target is 3′UTR of MMP-9.关键词
胰腺癌/骨桥蛋白/金属基质蛋白酶-9/3′UTRKey words
Pancreatic cancer/Osteopontin/Matrix metalloproteinase-9/3′UTR分类
医药卫生引用本文复制引用
周苏君,张国强,蒋超,蔡海,钟玉兵,高兴,王涛..骨桥蛋白对胰腺癌细胞金属基质蛋白酶-9转录后调控作用的研究[J].广西医学,2013,(12):1585-1587,1590,4.基金项目
江苏大学临床医学科技发展基金 （）
