广西医学Issue(2)：146-150,5.DOI:10.11675/j.issn.0253-4304.2014.02.03
HERG蛋白对胃癌细胞凋亡及细胞周期分布的影响
Effects of HERG Protein on Cell Cycle and Apoptosis of Gastric Cancer Cells
摘要
Abstract
Objective To explore the effects of human ether-a-go-go-related gene ( HERG ) protein on cell cycle and apoptosis of gastric cancer cells .Methods HERG-siRNA vector was constructed and transfected into gastric cancer cell SGC7901 ,G418 was applied to screening and cloning the transfected cells which were verified with HERG current and protein .Flow cytometry was used to assess the apoptosis of gastric cancer cells;Transmission electron microscope was used to observe the ultrastructure of gastric cancer cells ;The cell cycle distribution of gastric cancer cells was investigated by flow cytometry .Results HERG-siRNA eliminated the HERG current in gastric cancer cells and induced apoptosis in gastric cancer cells;There were typical changes of apoptosis under transmission electron microscope;HERG-siRNA inhibited gastric cancer cells entering into S phage from G 1 phage.Conclusion HERG-siRNA can inhibit HERG protein and potassium current as well as the progression of G 1/S of gastric cancer cells ,and induce apoptosis of gastric cancer cells .HERG -siRNA has potential anti-cancer effects on gastric cancer cells .关键词
胃癌/人eag相关基因蛋白/钾离子通道/细胞凋亡/细胞周期/钾电流Key words
Gastric cancer/Human ether-a-go-go-related gene/Potassium channel/Apoptosis/Cell cycle/Potassium current分类
医药卫生引用本文复制引用
邵晓冬,张永国,陈江,王金玲,郭晓钟..HERG蛋白对胃癌细胞凋亡及细胞周期分布的影响[J].广西医学,2014,(2):146-150,5.基金项目
国家自然科学基金资助项目 （）
