广西医学Issue(2)：187-190,4.DOI:10.11675/j.issn.0253-4304.2014.02.14
p53基因治疗对鼻咽癌患者 CD34标记的微血管密度和血小板计数的影响
Effect of p53 Gene Therapy on Microvessel Density Determined by CD34 and Platelet Count in Patients with Nasopharyngeal Carcinoma
摘要
Abstract
Objective To observe the effect of chemo-radiotherapy combined with recombinant human p 53 adenovirus(rAd-p53) on microvessel density(MVD) determined by CD34 and platelet(PLT) count in patients with nasopharyngeal carcinoma ,and to investigate its relationship with the prognosis .Methods Sixty-three patients with advanced nasopharyngeal carcinoma were randomly divided into two groups , the gene group ( 32 cases ) recieved rAd-p53 intratumoral injection and concurrent chemo-radiotherapy , the control group ( 31 cases ) recieved concurrent chemo-radiotherapy .The two-step immunohistochemical method was used to detect MVD ,which was marked by CD 34 in the primary tumor tissues of nasopharyngeal carcinoma in two groups ,and PLT was also detected.Results CD34-MVD of primary tumor tissues of nasopharyngeal carcinoma after treatment was significantly lower than that before treatment in the gene group(P<0.05),there was no significant difference in CD34-MVD before and after treatment in the control group (P>0.05).PLT significantly decreased after treatment in the two groups (all P<0.05),and the decrease in the gene group was more significant(P<0.05).The follow-up lasted for three years,there was no significant difference in the local recurrence and distant metastasis rates between experimental group and control group ( P>0.05);The distant metastasis rate in the increasing CD34-MVD group was significantly higher than that in the decreasing CD34-MVD group (38.5% vs. 13.5%,P<0.05),the 3-year overall survival ,disease-free survival in the increasing CD34-MVD group was lower than those in the decreasing CD34-MVD group(P<0.05);There was no significant difference in the local recurrence and distant metastasis rates ,3-year overall survival ,disease-free survival between increasing PLT group and decreasing PLT group (P>0.05).Conclusion CD34-MVD and PLT count decrease significantly after chemo-radiotherapy plus p53 gene therapy performed in patients with nasopharyngeal carcinoma .The over-expression of CD34-MVD could be a predictor of distant metastasis and poor prognosis of nasopharyngeal carcinoma .关键词
鼻咽癌/重组人p53腺病毒注射液/同步放化疗/微血管密度/血小板Key words
Nasopharyngeal carcinoma/Recombinant human p 53 adenovirus/Concurrent chemo-radiotherapy/Microvessel density/Platelet分类
医药卫生引用本文复制引用
周日晶,翁敬锦,司勇锋,韦海明,张政,兰桂萍,江河..p53基因治疗对鼻咽癌患者 CD34标记的微血管密度和血小板计数的影响[J].广西医学,2014,(2):187-190,4.基金项目
广西医药卫生科研课题 （）
