广西医学Issue(3)：273-276,4.DOI:10.11675/j.issn.0253-4304.2014.03.01
miR-9在宫颈癌组织中的表达及其靶基因的预测和生物信息学分析
Expression of miR-9 in Cervical Cancer Tissues and Bioinformatics Analysis of Predicted Target Genes
摘要
Abstract
Objective To investigate the expression of microRNA ( miRNA) in cervical cancer tissues and its predicted target genes to provide a theoretical basis for the study on the regulatory mechanism and biological functions of miR-9 .Methods MiRNA microarray was applied to detecting the miRNAs expressions in cervical caner tissues and normal cercvical tissues .The biological software was used to predict target genes of up-regulate miRNA-9.The miR-9 mimics were transected into cervical cancer cell line Hela ,and the mRNA microarrays was used to examine the expression of downstream genes of miR-9.The bioinformatics analysis of the target genes of miR-9 involved gene ontology and signal transduction pathway enrichment .Results Compared with normal cervical tissues ,there were 15 miRNAs up-expression and 10 miRNAs down-expression in cervical cancer tissues .miR-21 and miR-9 was significantly up-regulated while miR-376a and miR-218 were significantly down-regulated.The mRNA microarrays showed that 148 genes were down expression in miR-9,which also existed in network database ,and the genes set mainly located in biosynthetic and metabolic process as well as transcription and protein binding function .The KEGG pathway analysis demonstrated that the gene set mostly located in the regulation of actin cytoskeleton,sphingolipid metabolism and focal adhesion.Conclusion miR-9 expression significantly increases in cervical cancer tissues .The target genes set of miR-9 enriches in multiple biological function and process as well as tumor-related signaling pathways .关键词
宫颈癌/miR-9/靶基因/生物信息学Key words
Cervical cancer/miR-9/Target gene/Bioinformatics分类
医药卫生引用本文复制引用
张蔚,曾康康,莫祥兰,刘斐,胡晓霞..miR-9在宫颈癌组织中的表达及其靶基因的预测和生物信息学分析[J].广西医学,2014,(3):273-276,4.基金项目
国家自然科学基金资助项目（81060219）；广西自然科学基金资助项目 （）
