广西植物Issue(1)：10-14,5.DOI:10.3969/j.issn.1000-3142.2014.01.003
细叶云南松天然种源林遗传多样性的SSR分析
Analysis of genetic diversity of Pinus yunnanensis var. tenuifolia nature populations by SSR marker
摘要
Abstract
SSR markers were used to study genetic diversity of tree different nature populations of Pinus yunnanensis var.tenuifolia.The results showed that 13 sites and 37 alleles were detected.For every site,the average value of ob-servation allele number(A)was 2.85,polymorphism rate was 100% and the average values of effective number of al-leles (Ne)was 1.45.The average value of effective number of alleles was 1.447 within each population.P.yunnanen-sis var.tenuifolia had a low genetic diversity level among the three populations,the average observed heterozygosity (Ho)was 0.341 and the average expected gene heterozygosity(He)was 0.281.Nei’s gene diversities (h)at popula-tion level were 0.256 and 0.297.Shannon’s information indexes (I)at population level were 0.448 and 0.484.In addi-tion,the three populations deviated from Hardy-Weinberge quilibrium.The average coefficient gene differentiation (Gst)was 0.089.Most of variation appeared inner population and there was not gene differentiations among popula-tions.The range of gene flow of different gene polymorphism sites was 4.693-122.189,the average number was 1 1 .1 7 ,which indicated that the habitat of P .yunnanensis var.tenuifolia was limited and resulted in the limited natu-ral variation,but there were abundant gene exchanges between each population.关键词
细叶云南松/SSR分子标记/遗传多样性Key words
Pinus yunnanensis var.tenuifolia/SSR molecular marker/genetic diversity分类
农业科技引用本文复制引用
杨章旗,冯源恒,吴东山..细叶云南松天然种源林遗传多样性的SSR分析[J].广西植物,2014,(1):10-14,5.基金项目
八桂学者专项经费资助(松树资源培育及产业化关键技术创新) （松树资源培育及产业化关键技术创新）
