广西植物Issue(1):34-37,83,5.DOI:10.3969/j.issn.1000-3142.2014.01.007
中国西南地区特有水生植物海菜花微卫星引物开发
Microsatellites primer development for Ottelia acuminata (Hydrocharitaceae),a submerged macrophyte endemic to southwestern China
摘要
Abstract
Ottelia acuminata (Gagnepain)Dandy (Hydrocharitaceae),is a submerged monocot endemic to southwest-ern China.Using the fast isolation by AFLP of sequences containing repeats (FIASCO)protocol,nine polymorphic microsatellite loci were identified by the genotyping of forty-five individuals from three natural populations.The num-ber of alleles (NA)per locus within populations varied from one to three.The observed and expected heterozygosities ranged from 0.000 to 0.933 and 0.000 to 0.605,respectively.These microsatellite primers can be used in future studies on the phylogeography and ecological genetics of O.acuminata.关键词
水鳖科/海菜花/多态/微卫星Key words
Hydrocharitaceae/Ottelia acuminata/polymorphic/microsatellite分类
农业科技引用本文复制引用
卢然然,杨振艳,陶程程,陈绍田,纪运恒..中国西南地区特有水生植物海菜花微卫星引物开发[J].广西植物,2014,(1):34-37,83,5.基金项目
国家高技术研究发展计划项目(2010AA06Z301) (2010AA06Z301)
国家自然科学基金(31070297,31070198) (31070297,31070198)