华北农学报Issue(5)：29-33,5.
大豆胞囊线虫胁迫下不同抗性大豆杂交后代根系蛋白质组分析
Proteomic Analysis of Soybean with Different Resistance Differentially Expressed Proteins Induced by Heterodera glycines
摘要
Abstract
The cross was made between resistant variety Huipizhi Heidou ( ZDD2315 ) and susceptible variety Liaodou 15 to Heterodera glycines race 3 .Applying the F 4 separated population from the cross of Huipizhi Heidou and Liaodou 15 as test materials according to Bulked segregant analysis ( BSA ) to study differential proteomic .The total proteins were extracted by TCA/acetone ,two-dimensional gel electrophoresis and mass spectrometry were em-ployed to analyze proteins extracted from roots of soybean differentially expressed induced by Heterodera glycines. The results showed that there were 367 and 372 protein spots isolated in silver nitrate stained 2-D gels in resistant and sensitive samples,the 2-D gels were analyzed by ImageMaster 2D Platinum software,there were 6 special pro-tein spots ,13 twofold upper protein spots ,4 twofold lower protein spots in resistant samples ,4 special protein spots in sensitve samples .16 protein spots were identified by MALDI-TOF-MS and blast in NCBI , and 7 protein spots were from soybean ,11 protein spots weren′t identified due to the low scores .关键词
大豆/大豆胞囊线虫/双向电泳/质谱分析Key words
Soybean/Heterodera glycines/Two-dimensional gel electrophoresis/Mass spectrometry分类
农业科技引用本文复制引用
刘大伟,陈立杰,段玉玺..大豆胞囊线虫胁迫下不同抗性大豆杂交后代根系蛋白质组分析[J].华北农学报,2013,(5):29-33,5.基金项目
国家现代农业产业技术体系岗位科学家专项（CARS-04-PS13）；公益性行业科研专项（200903040）；东北农业大学博士启动基金项目 （）
