华北农学报Issue(5):34-37,4.
类猪圆环病毒 P1的感染及其分子特征
Porcine Circovirus like Virus P1 Infection and Its Molecular Characteristics
摘要
Abstract
Porcine circovirus like virus P1 is a new pathogen emerging in porcine and causing postweaning mul-tisystemic wasting syndrome ( PMWS)-like signs.P1 has the smallest genome up to now .To investigate the preva-lence and molecular characteristics of P1 , complete viral DNA genome sequences were amplified by PCR , cloned and sequenced .Bioinformatics was employed to analyze the nucleotide sequence and phylogeny .The results of this study indicated that the prevalence of P1 in the pig farms were quite high ,the nucleotide sequence similarity of P1 was 99 .1%to 100%,and it could be classified in two sublineage;Meanwhile ,distribution of P1 in samples were al-so detected by immunohistochemistry .关键词
类猪圆环病毒P1/PCR/免疫组化/分子特征Key words
Porcine circovirus like virus P1/PCR/Immunohistochemistry/Molecular characteristics分类
生物科学引用本文复制引用
温立斌,何孔旺,王凤芝,俞正玉,茅爱华,解建平..类猪圆环病毒 P1的感染及其分子特征[J].华北农学报,2013,(5):34-37,4.基金项目
国家自然科学基金项目(31272574;30972184);江苏省农业科技自主创新资金项目(CX(11)2060) ()