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类猪圆环病毒 P1的感染及其分子特征

温立斌 何孔旺 王凤芝 俞正玉 茅爱华 解建平

华北农学报Issue(5):34-37,4.
华北农学报Issue(5):34-37,4.

类猪圆环病毒 P1的感染及其分子特征

Porcine Circovirus like Virus P1 Infection and Its Molecular Characteristics

温立斌 1何孔旺 1王凤芝 2俞正玉 1茅爱华 1解建平1

作者信息

  • 1. 江苏省农业科学院 兽医研究所,农业部兽用生物制品工程技术重点实验室,国家兽用生物制品工程技术研究中心,江苏 南京 210014
  • 2. 南京农业大学,江苏 南京 210095
  • 折叠

摘要

Abstract

Porcine circovirus like virus P1 is a new pathogen emerging in porcine and causing postweaning mul-tisystemic wasting syndrome ( PMWS)-like signs.P1 has the smallest genome up to now .To investigate the preva-lence and molecular characteristics of P1 , complete viral DNA genome sequences were amplified by PCR , cloned and sequenced .Bioinformatics was employed to analyze the nucleotide sequence and phylogeny .The results of this study indicated that the prevalence of P1 in the pig farms were quite high ,the nucleotide sequence similarity of P1 was 99 .1%to 100%,and it could be classified in two sublineage;Meanwhile ,distribution of P1 in samples were al-so detected by immunohistochemistry .

关键词

类猪圆环病毒P1/PCR/免疫组化/分子特征

Key words

Porcine circovirus like virus P1/PCR/Immunohistochemistry/Molecular characteristics

分类

生物科学

引用本文复制引用

温立斌,何孔旺,王凤芝,俞正玉,茅爱华,解建平..类猪圆环病毒 P1的感染及其分子特征[J].华北农学报,2013,(5):34-37,4.

基金项目

国家自然科学基金项目(31272574;30972184);江苏省农业科技自主创新资金项目(CX(11)2060) ()

华北农学报

OA北大核心CSCDCSTPCD

1000-7091

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